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| Product Name | 6-HEX NHS Ester (6-Carboxy-2',4,4',5',7,7'-Hexachlorofluorescein Succinimidyl Ester) |
| Catalog No. | RERE-0002 |
| Description | 6-HEX, NHS is the succinimidyl ester of the hexachlorinated fluorescein 6-HEX. The chlorinated xanthene core gives a stable orange-yellow emission at 556 nm, and the reactive ester lets the fluorophore be attached straight to amine groups. Its documented home is nucleic acid sequencing and neighbouring labelling applications. |
| Intended Use | Fluorescent labelling of amines for nucleic acid sequencing and related research applications. |
| Principle / Technology | The NHS ester couples to primary amines, forming amide bonds that fix the 6-HEX fluorophore onto proteins, peptides or amine-modified oligonucleotides. |
| Detection Method | Fluorescence detection with excitation/emission of 535/556 nm. |
| Sample Type | Orange-yellow solid. |
| Performance Range / Specifications | CAS 2129651-79-0; molecular formula C25H9Cl6NO9; molecular weight 680.05 g/mol; appearance orange-yellow solid; purity ≥95%; Ex/Em 535/556 nm. |
| Specificity | Selective for primary amines; the hexachlorinated core distinguishes it spectrally from unmodified fluorescein. |
| Reaction Conditions / Protocol | Prepare a dry stock in anhydrous solvent, react with the amine-containing substrate at the chosen pH, then separate the labelled product from free dye. |
| Components / Formulation | One vial of dye; solvents and coupling buffers are supplied by the user. |
| Storage Conditions | Store at -25 to -15 °C, protected from light. |
| Shelf Life | 12 months under the recommended storage conditions. |
| Package Specifications | 1 mg, 5 mg or 25 mg |
| Product Form | Orange-yellow solid. |
| Quality Control | Released at purity ≥95%. |
| Key Features | - Hexachlorinated core gives stable 556 nm emission - Purity released at ≥95% - Direct amine coupling without extra activation steps - Three pack sizes from 1 mg to 25 mg - One-year shelf life protected from light |
| Purity | ≥95% |
| Activity / Unit Definition | Characterised by its spectral properties and amine-coupling reactivity; no unit definition applies. |
| Molecular Weight | CAS 2129651-79-0; molecular formula C25H9Cl6NO9; molecular weight 680.05 g/mol. |
| Source / Origin | Synthetic small-molecule fluorophore, supplied as a purified solid. |
| Expiration Date / Stability | Remains stable for the stated period provided the cold chain is not broken and light exposure is avoided. |
| Regulatory / Compliance | Supplied for laboratory research purposes. |
| Compatibility | Works on common fluorescence platforms; match the filter set to the excitation/emission values listed for this product. |
| Recommended Buffer System | No buffer is included; prepare anhydrous stock fresh before use. |
| Application Notes / Precautions | Handle protected from light, minimise freeze-thaw cycles by preparing aliquots, and verify that the detection channel matches the dye before use. |
| Batch-to-Batch Consistency | Consistency between lots is maintained by fixed production controls and release testing against specification. |
| Model Type | Amine-reactive fluorescent dye (succinimidyl ester) |
| Starting Material / Tissue Site | Synthesised 6-HEX fluorophore, esterified as the succinimidyl ester |
| Delivery Standard | Pack sizes 1 mg, 5 mg and 25 mg |
| Turnaround Time | dispatch timing is settled at purchase. |
| Readouts / Downstream Assays | Excitation/emission 535/556 nm on a suitable fluorescence channel. |
For research use only, not for clinical use.
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