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| Product Name | 5× SDS-PAGE Sample Loading Buffer |
| Catalog No. | PTR-HMM-0063 |
| Description | A concentrated protein loading buffer for SDS-PAGE containing SDS, glycerol, bromophenol blue tracking dye, and Tris-HCl buffer. The buffer denatures proteins and imparts negative charge for electrophoretic separation. |
| Intended Use | Denaturation and preparation of protein samples for loading onto SDS-PAGE gels; ensures uniform migration and visualization of protein bands during electrophoresis. |
| Principle / Technology | SDS binds to proteins in a constant mass ratio (~1.4 g SDS/g protein), imparting a uniform negative charge density that overwhelms intrinsic protein charge, enabling electrophoretic migration proportional to molecular weight. |
| Detection Method | Electrophoretic separation on SDS-PAGE gel; visual tracking of migration front by bromophenol blue dye |
| Sample Type | Protein samples from any biological source; compatible with purified proteins, cell lysates, and tissue extracts |
| Performance Range / Specifications | Working concentration: 1× loading buffer (dilute 5× stock with sample); recommended protein load: 10–50 μg per lane |
| Sensitivity / LOD | Visual detection of protein bands depends on subsequent staining method; loading buffer itself enables sample preparation only |
| Specificity | Compatible with all protein types; reducing agent (DTT or β-mercaptoethanol) should be added to reduce disulfide bonds for complete denaturation |
| Reaction Conditions / Protocol | Mix protein sample with 5× buffer (4:1 ratio), add reducing agent to 100 mM (if required), heat at 95–100°C for 5 minutes, cool briefly, centrifuge, load onto gel |
| Components / Formulation | 250 mM Tris-HCl pH 6.8, 10% (w/v) SDS, 50% (v/v) glycerol, 0.05% (w/v) bromophenol blue; reducing agent (DTT or β-mercaptoethanol) to be added by user |
| Storage Conditions | 2–8°C; aliquoting recommended for frequent use; protect from prolonged room temperature exposure |
| Shelf Life | 24 months from date of manufacture |
| Package Specifications | 10 mL (5× concentrate) |
| Product Form | Viscous blue liquid |
| Quality Control | Each lot tested by SDS-PAGE with molecular weight standards to confirm proper protein migration and dye front progression; pH verified (6.8±0.1); SDS activity validated |
| Key Features | Concentrated format for flexible sample volume adjustment; vibrant blue dye enables easy tracking of sample loading and electrophoretic front; stable formulation reduces preparation variability |
For research use only, not for clinical use.
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