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| Product Name | 3,3'-Diaminobenzidine (DAB) Tetrahydrochloride |
| Catalog No. | RERE-0045 |
| Description | DAB is the most widely used chromogenic substrate for horseradish peroxidase. In the presence of hydrogen peroxide the enzyme driven electron transfer builds a brown, insoluble reaction product that accumulates at the site of enzyme activity, giving sensitive and specific visual detection. The product is supplied as the tetrahydrochloride form, which dissolves readily in water, DMF and DMSO, and it is also known as a selenium reagent for spectrophotometric selenium determination. Because the coloured product is insoluble in ethanol, counterstains that dissolve in ethanol, such as methylene blue, methyl green and haematoxylin, can follow DAB development. |
| Intended Use | Chromogenic detection in immunohistochemistry, in situ hybridization and Western Blot; endogenous peroxidase demonstration in cells or tissue; spectrophotometric selenium content determination. |
| Principle / Technology | Horseradish peroxidase, with hydrogen peroxide as oxidant, converts DAB into a brown insoluble precipitate; colour intensity reflects enzyme labelled target abundance. |
| Detection Method | Peroxidase labelled antibodies, avidin enzyme conjugates and endogenous peroxidase activity |
| Sample Type | Tissue sections, cell preparations, blotted membranes and nucleic acid membranes |
| Performance Range / Specifications | High sensitivity and specificity; brown product stable through subsequent ethanol based counterstaining. |
| Specificity | Reacts specifically at peroxidase sites; product does not dissolve in alcohol, permitting ethanol compatible counterstains. |
| Reaction Conditions / Protocol | Prepare a 1% stock solution and store aliquoted at -20 °C. Before use dilute into buffer at pH 7.0 to 7.6, mix with hydrogen peroxide working solution and incubate the specimen until colour develops. |
| Storage Conditions | -20 °C, dry and protected from light |
| Shelf Life | 3 years |
| Package Specifications | 1 g |
| Product Form | Grey to dark red crystalline powder |
| Quality Control | Purity above 98% with lot to lot certificate of analysis. |
| Key Features | Tetrahydrochloride form for water solubility; multiple application modes; compatible with common counterstains. |
| Purity | >98% |
| Molecular Weight | 360.11 (anhydrous basis) |
| Regulatory / Compliance | For research use. |
| Compatibility | Soluble in water, DMF and DMSO; work solution used with hydrogen peroxide. |
| Application Notes / Precautions | DAB is a suspected carcinogen; wear gloves and handle solutions with appropriate containment. |
| Batch-to-Batch Consistency | Lot specific certificate of analysis supplied. |
For research use only, not for clinical use.
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