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| Product Name | 100 bp DNA Molecular Weight Marker |
| Catalog No. | NATR-HMM-0121 |
| Description | A ready-to-use DNA ladder containing 12 discrete double-stranded DNA fragments ranging from 100 bp to 3,000 bp. The marker provides accurate and precise molecular weight determination for double-stranded DNA fragments in agarose gel electrophoresis. |
| Intended Use | Molecular weight estimation of double-stranded DNA fragments separated by agarose gel electrophoresis in the 100–3,000 bp size range for PCR product analysis, restriction digestion characterization, and cloning verification. |
| Principle / Technology | DNA fragments migrate through agarose gel pores at rates inversely proportional to the log10 of their molecular weight under constant voltage. The reference bands of known sizes serve as calibration standards for interpolation of unknown fragment sizes. |
| Detection Method | Agarose gel electrophoresis with ethidium bromide, SYBR Safe, SYBR Green, or other DNA-binding fluorescent dyes; visualization by UV or blue-light transillumination |
| Sample Type | Double-stranded DNA samples separated on 1–3% agarose gels in TAE or TBE buffer |
| Performance Range / Specifications | Fragment sizes: 100, 200, 300, 400, 500, 600, 700, 800, 900, 1,000, 1,500, 2,000, and 3,000 bp; reference bands at 500 bp and 1,000 bp have increased intensity for easy orientation; recommended load: 5–10 μL per lane (0.5–1.0 μg total DNA) |
| Sensitivity / LOD | Bands as faint as 10 ng detectable with ethidium bromide staining; 2 ng with SYBR Safe or SYBR Gold superior staining methods |
| Specificity | Each fragment is a discrete double-stranded DNA species verified by sequencing; no detectable single-stranded or partially denatured DNA bands; minimal batch-to-batch migration variation |
| Reaction Conditions / Protocol | Thaw ladder at room temperature, mix gently by vortexing, load 5–10 μL per lane directly onto agarose gel; no heating or denaturation required; run gel at 5–8 V/cm for appropriate time based on gel concentration and fragment size range |
| Components / Formulation | Purified double-stranded DNA fragments in storage buffer (10 mM Tris-HCl pH 8.0, 1 mM EDTA, 5% glycerol, bromophenol blue and xylene cyanol FF tracking dyes); each fragment concentration adjusted for even band intensity |
| Storage Conditions | 2–8°C for up to 6 months; –20°C for long-term storage; stable for multiple freeze-thaw cycles; protect from prolonged exposure to temperatures >30°C |
| Shelf Life | 24 months at –20°C; 6 months at 2–8°C from date of manufacture |
| Package Specifications | 500 μL (100 applications at 5 μL per lane) and 5 × 500 μL bulk packaging |
| Product Form | Ready-to-use liquid solution; load directly on gel without additional loading buffer |
| Quality Control | Each lot verified by electrophoresis on 1.5% agarose: all bands visible, linear correlation of log(size) vs migration distance (R² ≥0.99); fragment identities confirmed by Sanger sequencing; no degradation after 7-day room temperature storage stability test |
| Key Features | Pre-mixed with loading dyes for direct use; reference bands at 500 and 1,000 bp for easy navigation; broad size range covers most PCR and cloning applications; compatible with all standard DNA stains; uniform band intensities for accurate quantification estimation |
For research use only, not for clinical use.
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