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| Product Name | Western Blot Transfer Buffer, Tris-Glycine, 10x |
| Catalog No. | ATR-B-YJL-0177 |
| Description | A ten-fold concentrated Tris-glycine transfer buffer with methanol addition required before use, optimized for electrophoretic transfer of proteins from SDS-PAGE gels to membrane supports. |
| Intended Use | Tank transfer of proteins from polyacrylamide gels to nitrocellulose or PVDF membranes in Western blotting workflows. |
| Principle / Technology | Tris-glycine provides ionic conductivity for protein migration; methanol (added by user) enhances protein binding to membranes and reduces gel swelling. |
| Detection Method | Western blot protein transfer verification with pre-stained markers, Ponceau S total protein staining |
| Sample Type | Proteins separated by SDS-PAGE, native PAGE, or IEF gels |
| Performance Range / Specifications | Transfer efficiency >80% for proteins 10–200 kDa at 100 V for 60 minutes |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Dilute 1:10 with water and add methanol to 20% v/v final concentration; pre-chill buffer; transfer at 100 V for 60 min or 30 V overnight. |
| Components / Formulation | Tris base 250 mM, glycine 1.92 M at 10x concentration; methanol added at time of use |
| Storage Conditions | 15–30°C |
| Shelf Life | 24 months |
| Package Specifications | 1 L, 4 L |
| Product Form | Liquid concentrate (10x), methanol not included |
| Quality Control | Conductivity and pH verification, transfer efficiency testing with standard protein ladder |
| Key Features | Standard Towbin transfer system; compatible with wet tank and semi-dry transfer; leaves methanol addition to user for flexibility |
For research use only, not for clinical use.
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