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| Product Name | Acetate Buffer, 3 M, pH 5.2, Molecular Biology Grade |
| Catalog No. | ATR-B-YJL-0193 |
| Description | A 3 M sodium acetate buffer at pH 5.2, prepared with DEPC-treated water and certified free of DNase, RNase, and protease activities. This high-concentration acetate buffer is a fundamental reagent for nucleic acid precipitation and purification workflows. When used at one-tenth volume with ethanol or isopropanol, the acetate ions neutralize the negative charge on the DNA phosphate backbone, facilitating efficient precipitation and pellet formation. |
| Intended Use | Precipitation of DNA and RNA from aqueous solution using ethanol or isopropanol in molecular biology workflows. |
| Principle / Technology | Sodium acetate charge neutralization of nucleic acid phosphate backbone enabling alcohol precipitation |
| Detection Method | Spectrophotometric quantification or gel electrophoresis of recovered nucleic acid |
| Sample Type | DNA or RNA in aqueous solution from enzymatic reactions, extraction procedures, or column eluates |
| Performance Range / Specifications | Final concentration in precipitation: 0.3 M sodium acetate with 2.5 volumes ethanol or 0.7 volumes isopropanol |
| Sensitivity / LOD | Typical recovery: ≥90% for DNA fragments ≥100 bp; ≥80% for oligonucleotides ≥20 nt |
| Specificity | Broad nucleic acid precipitation; no sequence or base composition bias |
| Reaction Conditions / Protocol | Add 1/10 volume 3 M sodium acetate, mix; add 2.5 volumes cold 100% ethanol; incubate at –20 °C for ≥30 min; centrifuge 15 min at 4 °C; wash with 70% ethanol |
| Components / Formulation | 3 M sodium acetate solution, pH 5.2, prepared with DEPC-treated water |
| Storage Conditions | Room temperature |
| Shelf Life | 24 months at room temperature |
| Package Specifications | 100 mL, 500 mL |
| Product Form | Liquid solution, clear and colorless |
| Quality Control | DNase, RNase, and protease testing negative per lot; pH verified 5.2 ± 0.1; sterile-filtered |
| Key Features | Ready-to-use high-concentration format eliminates the need for weighing and pH-adjusting solid sodium acetate |
| Purity | ≥99%; DNase/RNase free; sterile-filtered 0.22 µm |
| Concentration | As specified on product label |
| Activity / Unit Definition | pH buffering capacity within ±0.05 pH units of stated value |
| Molecular Weight | Not applicable — inorganic/organic buffer salts |
| Source / Origin | Synthetic, prepared from analytical grade reagents |
| pH Range / Optimal pH | As stated in product specification |
| Shipping Conditions | Ambient temperature; protect from extreme heat |
| Expiration Date / Stability | 12–24 months at recommended storage temperature |
| Regulatory / Compliance | For research use and IVD manufacturing; ISO 13485 quality system |
| Compatibility | Compatible with standard biochemical and cell biology applications |
| Recommended Buffer System | Use as directed; compatible with biological assay systems |
| Application Notes / Precautions | Avoid contamination; use aseptic technique for sterile products; verify pH before critical applications |
| Batch-to-Batch Consistency | pH within ±0.05 units; conductivity within ±5% of reference lot |
For research use only, not for clinical use.
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