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| Product Name | Ultra-Fidelity Universal-Annealing PCR Master Mix |
| Catalog No. | MDKPK-0001 |
| Description | A 2× hot-start high-fidelity PCR master mix formulated for universal annealing conditions, rapid extension and amplification of conventional or challenging templates. The premix contains DNA polymerase, dNTPs, magnesium ions and other essential reaction components. |
| Intended Use | high-fidelity PCR for molecular cloning, gene identification and gene synthesis |
| Principle / Technology | Hot-start high-fidelity DNA polymerase amplification in a ready-to-use 2× master mix. |
| Detection Method | Endpoint PCR followed by electrophoretic analysis. |
| Sample Type | Conventional DNA templates, crude samples and high-GC or high-AT templates. |
| Performance Range / Specifications | Extension: 5–10 sec/kb; supports fragments up to 40 kb; compatible GC range reported as 24%–97%. |
| Specificity | Approximately 200-fold higher fidelity than Taq DNA polymerase. |
| Components / Formulation | High-fidelity DNA polymerase, dNTPs, Mg2+ and PCR buffer in a 2× premix. |
| Storage Conditions | −30 to −15°C |
| Package Specifications | 1 mL; 5 × 1 mL; 15 × 1 mL |
| Product Form | 2× PCR master mix |
| Key Features | Universal annealing program; rapid extension; very high fidelity; broad template compatibility; long-fragment amplification. |
| Shipping Conditions | Ship at 0°C or below. |
| Compatibility | Routine templates, crude lysates and complex high-GC/high-AT systems. |
For research use only, not for clinical use.
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