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| Product Name | 2× Rapid Taq PCR Master Mix with Loading Dye |
| Catalog No. | MDKPK-0008 |
| Description | A ready-to-use 2× PCR mix formulated for fast Taq-based amplification. The premix combines the amplification reagents with electrophoresis buffer and green loading dye, allowing PCR products to be loaded directly onto a gel after cycling. |
| Intended Use | Rapid PCR, colony PCR and genotyping. |
| Principle / Technology | Fast Taq DNA polymerase amplification in a 2× premixed format containing electrophoresis buffer and green loading dye. |
| Detection Method | Endpoint PCR followed by direct agarose gel electrophoresis. |
| Sample Type | Human genomic DNA, human cDNA, mouse genomic DNA, wheat genomic DNA, rice genomic DNA, bacterial cultures, bacterial colonies and lambda DNA. |
| Performance Range / Specifications | Typical extension speed is 15 sec/kb; fragments up to 1 kb can be amplified at 1 sec/kb. Demonstrated with 1–3.3 kb targets. |
| Specificity | Designed to provide strong specificity and high amplification yield. |
| Reaction Conditions / Protocol | Add primers and template directly to the premix. No ice setup is required. Use an extension time of 15 sec/kb; for targets up to 1 kb, 1 sec/kb may be used. |
| Components / Formulation | 2× rapid Taq PCR master mix containing Taq DNA polymerase, dNTPs, optimized PCR buffer, electrophoresis buffer and green loading dye. |
| Storage Conditions | Store at −20°C. After thawing, the product may be kept at 4°C for up to 3 months. |
| Package Specifications | 5 × 1 mL; 15 × 1 mL; 50 × 1 mL; 10 × 5 mL. |
| Product Form | 2× PCR master mix with loading dye. |
| Key Features | Rapid extension; direct gel loading; simple two-component setup with primers and template; stable after 50 freeze-thaw cycles; high specificity and yield. |
| Compatibility | Suitable for T-vector cloning because the PCR products carry 3′-A overhangs. |
| Application Notes / Precautions | PCR products contain 3′-A overhangs and can be used for T-vector cloning. |
For research use only, not for clinical use.
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