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| Product Name | TUNEL FITC Apoptosis Detection Kit |
| Catalog No. | CAAK-0003 |
| Description | A green-fluorescence TUNEL assay for detecting DNA fragmentation associated with apoptosis in cultured cells and tissue sections. |
| Intended Use | Detection of apoptotic cells in paraffin sections, frozen sections, adherent cells and suspension cells. |
| Principle / Technology | Recombinant terminal deoxynucleotidyl transferase catalyzes incorporation of FITC-12-dUTP at exposed 3′-OH DNA ends. An optimized ratio of labeled nucleotide to unlabeled dNTP improves signal-to-noise and labeling efficiency. |
| Detection Method | Fluorescence microscopy or flow cytometry. |
| Sample Type | Paraffin sections, frozen sections, adherent cultured cells and suspension cultured cells. |
| Performance Range / Specifications | Available for 20, 50 or 100 reactions. |
| Reaction Conditions / Protocol | Fix and permeabilize the sample, perform TdT-mediated fluorescent nucleotide labeling, wash thoroughly and analyze by fluorescence microscopy or flow cytometry. |
| Components / Formulation | Recombinant TdT enzyme and FITC-12-dUTP fluorescent labeling mixture with optimized unlabeled nucleotide content. |
| Storage Conditions | Store at -30 to -15°C. Protect the FITC-12-dUTP labeling mixture from light. |
| Package Specifications | 20 reactions; 50 reactions; 100 reactions. |
| Product Form | Green-fluorescence TUNEL apoptosis kit. |
| Key Features | Optimized signal-to-noise; high-activity recombinant TdT; direct fluorescent detection; suitable for cultured cells and tissue sections. |
| Compatibility | Fluorescence microscopes and flow cytometers. |
| Application Notes / Precautions | Include positive and negative controls, use appropriate fixation and permeabilization, keep samples moist and protect fluorescent reagents from light. |
For research use only, not for clinical use.
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