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| Product Name | Annexin V-FITC/PI Apoptosis Detection Kit |
| Catalog No. | CAAK-0004 |
| Description | A dual-fluorescence apoptosis kit using FITC-labeled Annexin V and propidium iodide for quantitative classification of cell-death stages. |
| Intended Use | Detection and quantification of apoptosis in adherent and suspension cultured cells. |
| Principle / Technology | Annexin V-FITC binds phosphatidylserine exposed during early apoptosis, while propidium iodide labels DNA in cells with compromised membranes. |
| Detection Method | Flow cytometry. |
| Sample Type | Adherent and suspension cultured cells. |
| Performance Range / Specifications | Available for 50 or 100 reactions. |
| Specificity | Dual staining separates viable, early-apoptotic and late-apoptotic or necrotic cells. |
| Reaction Conditions / Protocol | Stain harvested cells with Annexin V-FITC and propidium iodide and analyze with appropriate compensation and controls. |
| Components / Formulation | Annexin V-FITC and propidium iodide staining reagents. |
| Storage Conditions | Store at 4-8°C. For prolonged storage, Annexin V-FITC may be kept at -20°C. |
| Package Specifications | 50 reactions; 100 reactions. |
| Product Form | Green-fluorescence apoptosis assay kit. |
| Key Features | Green fluorescent Annexin V label; PI-based membrane-integrity discrimination; quantitative early and late apoptosis analysis. |
| Compatibility | Flow cytometers supporting FITC and PI channels. |
| Application Notes / Precautions | Protect fluorescent reagents from light and include unstained and single-color controls. |
For research use only, not for clinical use.
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