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| Product Name | Tris-HCl electrophoresis buffer raw material for protein separation, CAS 1185-53-1, purity >99% |
| Catalog No. | ATR-B-YJL-0284 |
| Description | Tris hydrochloride (Tris-HCl) is a widely used biological buffer that offers broad and convenient application in electrophoresis. The primary function of an electrophoresis buffer is to maintain a stable pH, because the charge properties of biomolecules strongly influence their mobility in an electric field, and any shift in pH alters that charge and therefore the migration behavior. Tris-HCl is frequently employed as the pH buffer of choice in protein electrophoresis, delivering a stable environment that supports reliable protein separation and identification. It combines good cost-effectiveness with strong protection of biomolecules against damage during electrophoretic runs, and it is suitable for a wide range of electrophoresis formats. |
| Intended Use | Used as a pH buffer in protein electrophoresis and related electrophoretic techniques to maintain stable pH conditions for biomolecule separation and analysis. |
| Principle / Technology | The core function of an electrophoresis buffer is to keep the pH of the system constant, since pH changes alter the net charge of biomolecules and consequently their mobility in an electric field. Tris-HCl stabilizes pH by reversibly binding and releasing hydrogen ions: it can dissociate to release protons or associate with free protons as needed, thereby counteracting pH fluctuations and maintaining a stable environment throughout the electrophoretic run. |
| Package Specifications | 25 kg/drum |
| Key Features | Good cost-effectiveness for routine electrophoresis work Strong protective effect on biomolecules, preventing damage during electrophoresis Wide applicability across different types of electrophoresis experiments |
| Purity | >99% |
| Application Notes / Precautions | Protein electrophoresis: used as a pH buffer to maintain a stable pH during separation Isoelectric focusing (IEF): ensures target proteins focus precisely at their isoelectric points SDS-polyacrylamide gel electrophoresis (SDS-PAGE): provides a stable pH environment for protein separation and identification Two-dimensional gel electrophoresis: supports reproducible high-resolution protein separation |
| Alias | Tris-HCl |
| CAS Number | 1185-53-1 |
| Other Name | TRIS hydrochloride |
For research use only, not for clinical use.
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