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| Product Name | Tris-HCl buffer with CAS 1185-53-1 for low-osmolarity buffer configuration in cell-sensitive workflows |
| Catalog No. | ATR-B-YJL-0301 |
| Description | Tris-HCl buffer is one of the most commonly used laboratory buffers, valued for its well-defined pKa of 8.1 at 25°C and an effective buffering range of pH 7.0-9.0. Its relatively low osmotic pressure makes it suitable for osmolarity-sensitive systems such as cell-based experiments. The buffer can be autoclaved and stored at room temperature, and it is widely applied in DNA/RNA extraction, protein purification, and enzyme reaction systems. |
| Intended Use | Laboratory buffer preparation for DNA/RNA extraction, protein purification, enzyme reaction systems, and osmolarity-sensitive experiments. |
| Reaction Conditions / Protocol | Adjust pH with hydrochloric acid at the intended working temperature, since Tris buffer pH is temperature-sensitive. The buffer can be autoclaved, but pH may shift slightly afterward; confirm the pH again once cooled. |
| Storage Conditions | Store prepared buffer at room temperature, protected from microbial contamination; add a preservative or filter-sterilize when necessary. |
| Package Specifications | 25 kg per drum |
| Key Features | Low osmotic pressure suitable for osmolarity-sensitive systems such as cell experiments; autoclavable; effective buffering across pH 7.0-9.0. |
| Purity | ≥99% |
| Molecular Weight | 157.60 |
| pH Range / Optimal pH | Effective buffering range pH 7.0-9.0; pKa 8.1 at 25°C |
| Application Notes / Precautions | pKa is 8.1 at 25°C with an effective buffering range of pH 7.0-9.0; perform pH adjustment at the target temperature; verify pH after autoclaving; prevent microbial contamination during storage. |
| Molecular Formula | C4H11NO3·HCl |
| Appearance | White crystalline powder |
| CAS Number | 1185-53-1 |
| Osmotic Pressure | Low osmotic pressure, suitable for osmolarity-sensitive experimental systems such as cell experiments |
| Sterilization | Autoclavable; pH may shift slightly after sterilization, so re-confirm pH after cooling |
For research use only, not for clinical use.
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