- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Tris-HCl Buffer, 1 M, pH 8.0 |
| Catalog No. | ATR-B-YJL-0166 |
| Description | A 1.0 molar Tris-HCl solution calibrated to pH 8.0, optimized for alkaline lysis plasmid extraction protocols and general DNA manipulation procedures. |
| Intended Use | Buffering agent for plasmid miniprep procedures, DNA storage, restriction enzyme digests, and alkaline phosphatase reactions. |
| Principle / Technology | Tris base protonation equilibrium providing stable pH at alkaline range near the pKa value. |
| Detection Method | pH electrode |
| Sample Type | Plasmid DNA, genomic DNA, restriction fragments, PCR products |
| Performance Range / Specifications | pH 7.8–8.2 at working dilution |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Use at 10–50 mM final concentration in enzymatic reactions; component of TE buffer at 10 mM with 1 mM EDTA. |
| Components / Formulation | Tris base in ultrapure water, pH adjusted with concentrated HCl |
| Storage Conditions | 15–30°C |
| Shelf Life | 36 months |
| Package Specifications | 100 mL, 500 mL, 1 L |
| Product Form | Liquid concentrate |
| Quality Control | pH ±0.05, DNase/RNase/protease free, conductivity verification |
| Key Features | Nuclease free; standard for molecular biology; compatible with EDTA for metal chelation in TE buffer |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
