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| Product Name | Tris-HCl Buffer, 1 M, pH 7.5 |
| Catalog No. | ATR-B-YJL-0165 |
| Description | A 1.0 molar Tris-HCl stock solution adjusted to pH 7.5 with hydrochloric acid, widely used as a buffering component in enzymatic and molecular biology reactions. |
| Intended Use | Buffering agent for DNA and RNA manipulation, enzyme reactions, protein purification, and as a component in electrophoresis and chromatography buffers. |
| Principle / Technology | Tris (tris(hydroxymethyl)aminomethane) acts as a primary amine base that establishes equilibrium with its protonated form, providing effective buffering near its pKa of 8.07 at 25°C. |
| Detection Method | pH electrode |
| Sample Type | Nucleic acids, proteins, enzymes, PCR components |
| Performance Range / Specifications | pH 7.3–7.7 at working dilution, effective buffering range pH 7.0–9.0 |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Dilute to desired molarity in final reaction; typical working concentration 10–100 mM. |
| Components / Formulation | Tris base dissolved in ultrapure water, titrated with concentrated HCl to pH 7.5 |
| Storage Conditions | 15–30°C |
| Shelf Life | 36 months |
| Package Specifications | 100 mL, 500 mL, 1 L |
| Product Form | Liquid concentrate |
| Quality Control | pH verification, concentration by titration, DNase/RNase testing, heavy metal analysis |
| Key Features | Molecular biology grade; nuclease free; multiple pH options available; compatible with most enzymatic reactions |
For research use only, not for clinical use.
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