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| Product Name | Tris-EDTA (TE) Buffer, pH 8.0, 100x |
| Catalog No. | ATR-B-YJL-0188 |
| Description | A concentrated Tris-EDTA buffer stock for DNA and RNA storage and dilution, providing nuclease inhibition through metal chelation and stable pH for nucleic acid stability. |
| Intended Use | Storage and dilution buffer for purified DNA and RNA, resuspension of oligonucleotides, and preparation of molecular biology working solutions. |
| Principle / Technology | Tris maintains pH 8.0 to minimize depurination and hydrolysis; EDTA chelates Mg2+ and other divalent cations required for nuclease activity, protecting nucleic acids from degradation. |
| Detection Method | Spectrophotometric A260/A280 ratio, agarose gel integrity check |
| Sample Type | Plasmid DNA, genomic DNA, PCR products, synthetic oligonucleotides, total RNA preparations |
| Performance Range / Specifications | DNA storage stability >5 years at -20°C in TE buffer |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Dilute 1:100 with ultrapure water; use for DNA/RNA elution, storage, or dilution. |
| Components / Formulation | Tris-HCl 1 M, EDTA 0.1 M, pH 8.0 at 100x |
| Storage Conditions | 15–30°C |
| Shelf Life | 36 months |
| Package Specifications | 100 mL, 500 mL |
| Product Form | Liquid concentrate (100x) |
| Quality Control | pH verification, DNase/RNase free, heavy metal testing |
| Key Features | Nuclease free; molecular biology grade; 100x for economical dilution; standard for nucleic acid storage |
For research use only, not for clinical use.
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