- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Tris-Buffered Saline with Tween 20 (TBST), 10x Concentrate |
| Catalog No. | ATR-B-YJL-0173 |
| Description | A ten-fold concentrated wash buffer containing Tris-buffered saline with 0.5% Tween 20 surfactant, designed for washing steps in Western blotting, ELISA, and protein array procedures. |
| Intended Use | Wash buffer for removing unbound antibodies and reagents during immunoassay procedures, with surfactant to reduce background binding. |
| Principle / Technology | Tris maintains alkaline pH optimal for antibody-antigen interactions; Tween 20 detergent disrupts weak hydrophobic interactions and reduces non-specific binding. |
| Detection Method | pH electrode, surface tension measurement |
| Sample Type | Blot membranes, ELISA plates, protein microarrays, bead-based assays |
| Performance Range / Specifications | pH 7.4–7.6 after 1:10 dilution |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Dilute 1 part concentrate with 9 parts deionized water; use 3–5 washes of 5 minutes each for Western blotting. |
| Components / Formulation | Tris base, sodium chloride, Tween 20 in ultrapure water, pH adjusted with HCl |
| Storage Conditions | 15–30°C |
| Shelf Life | 24 months |
| Package Specifications | 500 mL, 1 L, 4 L |
| Product Form | Liquid concentrate |
| Quality Control | pH verification, surfactant activity testing, DNase/RNase free |
| Key Features | Ready to dilute; consistent lot-to-lot Tween 20 concentration; nuclease free; economical concentrate format |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
