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| Product Name | Tricine zwitterionic biological buffer raw material, CAS 5704-04-1, purity >99% |
| Catalog No. | ATR-B-YJL-0293 |
| Description | Tricine (CAS 5704-04-1) is a zwitterionic buffer commonly used in biological and biochemical research. It has the molecular formula C6H13NO5 and a molecular weight of 179.17, with a melting point of 186–188 °C and an estimated boiling point of approximately 311.69 °C. Its density is 1.05 g/mL at 20 °C. The pKa of Tricine is 8.1 at 25 °C, and a 1 M aqueous solution exhibits a pH between 7.4 and 8.8, making it well suited for buffering in the slightly alkaline range. |
| Intended Use | Used as a biological buffer raw material to maintain the stability and activity of biomolecules such as proteins, enzymes and DNA, and as a buffering component in biochemical, immunological and molecular biology experiments. |
| Package Specifications | 25 kg/drum |
| Product Form | Solid buffer raw material |
| Key Features | Effective buffering range of pH 7.4–8.8 (1 M aqueous solution), with a pKa of 8.1 at 25 °C. Well suited as an electrophoresis buffer, particularly for the separation and qualitative analysis of small proteins and peptides. Applicable as an eluent in chromatographic analysis and in synthesis and separation processes. Can serve as a carrier in radioimmunoassays, facilitating the labeling of antigens and antibodies. |
| Purity | >99% |
| Molecular Weight | 179.17 |
| pH Range / Optimal pH | pH 4.0–6.0 (general); 1 M aqueous solution: pH 7.4–8.8 |
| Application Notes / Precautions | Commonly selected for electrophoretic separation of low-molecular-weight proteins and peptides. Suitable for immunology and molecular biology workflows requiring stable mildly alkaline conditions. Store and handle according to standard laboratory chemical practices. |
| Molecular Formula | C6H13NO5 |
| Alias | Tricine; N-[Tris(hydroxymethyl)methyl]glycine |
| CAS Number | 5704-04-1 |
| pKa | 8.1 (at 25 °C) |
| Density | 1.05 g/mL at 20 °C |
| Melting Point | 186–188 °C |
| Indicator Transition Range | pH 7.4–8.8 |
| Boiling Point | approx. 311.69 °C (estimated) |
For research use only, not for clinical use.
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