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| Product Name | Tricine-SDS-PAGE Running Buffer, 10× Concentrate |
| Catalog No. | ATR-B-YJL-0192 |
| Description | A 10× running buffer concentrate formulated for Tricine-SDS-PAGE, a modified electrophoretic system optimized for resolving low molecular weight proteins and peptides in the 1–100 kDa range. The Tricine-based buffer system provides superior resolution of small polypeptides compared to conventional glycine-SDS-PAGE by slowing the migration rate of the chloride leading ion relative to the Tricine trailing ion. |
| Intended Use | High-resolution electrophoretic separation of low molecular weight proteins and peptides by Tricine-SDS-PAGE. |
| Principle / Technology | Tricine-based discontinuous buffer system for SDS-PAGE with modified Kohlrausch regulating function |
| Detection Method | Coomassie staining, silver staining, or Western blot detection of separated products |
| Sample Type | Purified proteins, cell lysates, peptide mixtures with molecular weights from 1–100 kDa |
| Performance Range / Specifications | Resolution range: linear separation of 1–100 kDa species with optimal resolution for 1–30 kDa peptides |
| Sensitivity / LOD | Detection of peptides as small as 1 kDa with clear band separation |
| Specificity | Buffer specifically formulated for Tricine-SDS-PAGE; not interchangeable with glycine-SDS-PAGE buffer |
| Reaction Conditions / Protocol | Dilute to 1×; use as cathode (top) buffer; anode (bottom) buffer typically 0.2 M Tris-HCl, pH 8.9; run at constant voltage 125 V until dye front reaches bottom |
| Components / Formulation | 10× Tricine-SDS cathode buffer concentrate (1 M Tris, 1 M Tricine, 1% SDS, pH 8.25) |
| Storage Conditions | Room temperature |
| Shelf Life | 18 months at room temperature |
| Package Specifications | 500 mL, 1 L |
| Product Form | Liquid concentrate |
| Quality Control | Conductivity and pH verified per batch; resolution validated by separation of pre-stained peptide molecular weight markers |
| Key Features | Superior separation of small peptides compared to standard glycine-SDS-PAGE, with sharp bands in the 5–20 kDa range |
| Purity | ≥99%; DNase/RNase free; sterile-filtered 0.22 µm |
| Concentration | As specified on product label |
| Activity / Unit Definition | pH buffering capacity within ±0.05 pH units of stated value |
| Molecular Weight | Not applicable — inorganic/organic buffer salts |
| Source / Origin | Synthetic, prepared from analytical grade reagents |
| pH Range / Optimal pH | As stated in product specification |
| Shipping Conditions | Ambient temperature; protect from extreme heat |
| Expiration Date / Stability | 12–24 months at recommended storage temperature |
| Regulatory / Compliance | For research use and IVD manufacturing; ISO 13485 quality system |
| Compatibility | Compatible with standard biochemical and cell biology applications |
| Recommended Buffer System | Use as directed; compatible with biological assay systems |
| Application Notes / Precautions | Avoid contamination; use aseptic technique for sterile products; verify pH before critical applications |
| Batch-to-Batch Consistency | pH within ±0.05 units; conductivity within ±5% of reference lot |
For research use only, not for clinical use.
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