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| Product Name | TE Buffer, 1x, pH 8.0, Nuclease-Free, 0.1 um Sterile Filtered |
| Catalog No. | ATR-B-YJL-0199 |
| Description | Ready-to-use 1x TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0) prepared with ultrapure water, DEPC-treated, autoclaved, and 0.1 um sterile-filtered. TE buffer is the most widely used buffer for dissolving and storing nucleic acids (DNA and RNA), providing a stable, nuclease-free, slightly alkaline environment. The Tris component buffers the solution at pH 8.0, minimizing acid-catalyzed depurination and beta-elimination of abasic sites in DNA. The EDTA component (1 mM) chelates divalent cations (Mg2+, Ca2+, Mn2+) that are essential cofactors for most nucleases (DNases and RNases), thereby protecting nucleic acids from enzymatic degradation during storage. This formulation uses ultrapure components tested for the absence of DNase, RNase, protease, and endotoxin, making it suitable for sensitive downstream applications including qPCR, NGS library preparation, and single-cell genomics. |
| Intended Use | Dissolution and long-term storage of: genomic DNA, plasmid DNA, PCR products, cDNA, oligonucleotides, and total RNA at -20 C or -80 C; dilution buffer for nucleic acid standards and samples prior to spectrophotometric (NanoDrop) or fluorometric (Qubit) quantification; dilution buffer for enzymes, primers, and probes in molecular biology workflows; washing/elution buffer in nucleic acid purification procedures; blank/reference solution for UV spectrophotometry at 260 and 280 nm. |
| Principle / Technology | Tris (pKa 8.07 at 25 C) provides buffering capacity at pH 8.0, which is near-optimal for DNA stability — alkaline pH minimizes depurination (acid-catalyzed cleavage of purine bases) and strand breakage. EDTA chelates Mg2+ (Kd ~10^5-10^6 M^-1 at pH 8.0) and other divalent metals, effectively inhibiting DNase I (requires Mg2+ and Ca2+), DNase II (requires Mg2+ and Mn2+), and most RNases (require Mg2+). At 1 mM EDTA, sufficient chelation capacity is provided for typical divalent metal contamination in buffer-grade reagents while maintaining compatibility with downstream enzymatic reactions (excess EDTA can interfere with Mg2+-dependent polymerases and restriction enzymes if carryover is high). |
| Detection Method | Ready-to-use 1x solution — no dilution required. Use directly for: resuspending dried/lyophilized nucleic acid pellets; diluting concentrated nucleic acid stocks; preparing oligonucleotide working solutions; performing blank measurement on spectrophotometer before sample reading. For long-term storage of nucleic acids at -20 C or -80 C, aliquot TE-dissolved samples to minimize freeze-thaw cycles. |
| Sample Type | Ready-to-use buffer; equilibrate to RT before use; use aseptic technique when aliquoting to maintain sterility. |
| Performance Range / Specifications | Composition: 10 mM Tris-HCl, 1 mM EDTA, pH 8.0 +/-0.05; conductivity: 0.6-0.8 mS/cm at 25 C; A260 <0.01, A280 <0.01 (against water blank); nuclease-free: no detectable DNase or RNase; endotoxin: <0.005 EU/mL; sterility: <0.1 CFU/mL. |
| Sensitivity / LOD | A260/A280 blank reading: typically A260 <0.005, A280 <0.005 (High Sensitivity mode); absence of nuclease activity verified by incubation with high molecular weight DNA (lambda, 48.5 kb) and RNA for 24 h at 37 C — no degradation detected by agarose gel. |
| Specificity | Formulated specifically for nucleic acid storage and handling; Tris at pH 8.0 specifically chosen for DNA stability; EDTA at 1 mM specifically chosen as nuclease inhibitor with minimal downstream interference; not suitable as an enzyme reaction buffer (lacks Mg2+ and reducing agents). |
| Reaction Conditions / Protocol | Storage conditions: DNA in TE stable at -20 C for years, at -80 C for decades, at 2-8 C for months (plasmid), at RT for days to weeks (genomic DNA in TE may degrade at RT over extended periods). Repeated freeze-thaw cycles of DNA in TE cause progressive shearing of high molecular weight DNA. |
| Components / Formulation | 1x TE Buffer, pH 8.0: 10 mM Tris-HCl (from Tris base adjusted with HCl), 1 mM EDTA (from EDTA disodium dihydrate), in ultrapure water (DEPC-treated, then autoclaved to destroy DEPC), 0.1 um sterile-filtered into sterile bottle. |
| Storage Conditions | Store at RT (15-25 C); shelf life 24 months unopened; after opening, store at RT and use within 6 months with aseptic technique; if microbial contamination is suspected (turbidity, odor), discard. |
| Shelf Life | 24 months from date of manufacture at RT; after opening, 6 months with aseptic technique. |
| Package Specifications | 100 mL, 500 mL, 1 L, 4 L. |
| Product Form | Clear, colorless sterile liquid; no visible particulates. |
| Quality Control | Each lot: pH 7.98-8.02; conductivity 0.6-0.8 mS/cm; A260 <0.01, A280 <0.01; DNase: absent (lambda DNA integrity after 24 h at 37 C); RNase: absent (rRNA integrity after 1 h at 37 C); protease: absent (azocasein assay); endotoxin: <0.005 EU/mL; sterility: bacterial and fungal count <0.1 CFU/mL. |
| Key Features | Ready-to-use 1x TE (no dilution needed); pH 8.0 optimized for DNA stability; nuclease-free (DNase and RNase tested); DEPC-treated and autoclaved; 0.1 um sterile filtered; ultra-low endotoxin (<0.005 EU/mL); A260/A280 blank <0.01. |
| Purity | All components: molecular biology grade, >=99% purity; water: ultrapure (18.2 MOhm-cm), DEPC-treated, autoclaved; nuclease-free certified. |
| Concentration | 1x working solution: 10 mM Tris-HCl, 1 mM EDTA, pH 8.0. |
| Activity / Unit Definition | Not applicable — buffer solution; nuclease inhibitor function via EDTA chelation of divalent cations. |
| Molecular Weight | Tris base: 121.14 g/mol; Tris-HCl: 157.60 g/mol; EDTA disodium dihydrate: 372.24 g/mol. |
| Source / Origin | Synthetic chemicals (Tris, EDTA from chemical synthesis); water: ultrapure deionized, DEPC-treated, autoclaved; no animal-derived components. |
| pH Range / Optimal pH | pH 8.0 +/-0.05 at 25 C; Tris effective buffer range pH 7.2-9.0; solution pH stable for product shelf life. |
| Shipping Conditions | Ambient temperature. |
| Expiration Date / Stability | 24 months at RT unopened; after opening, 6 months at RT; do not freeze 1x TE (expansion may cause bottle rupture or leakage). |
| Regulatory / Compliance | For research use only; not for diagnostic or therapeutic use. Not for human or animal injection. |
| Compatibility | Compatible with DNA and RNA storage; compatible with spectrophotometric (NanoDrop, UV-Vis) and fluorometric (Qubit, PicoGreen, RiboGreen) quantification methods. Compatible with most downstream enzymatic reactions at low carryover volumes: PCR (1-2 uL TE in 25-50 uL reaction typically acceptable), restriction digestion (EDTA at <0.1 mM final does not interfere), ligation (EDTA may chelate Mg2+ in ligation buffer — keep TE volume <10% of reaction), and NGS library preparation. For single-cell and ultra-low-input applications, use low-EDTA TE (10 mM Tris, 0.1 mM EDTA) or 10 mM Tris-HCl pH 8.0 without EDTA to minimize EDTA carryover in enzymatic reactions. |
| Recommended Buffer System | 1x TE Buffer: 10 mM Tris-HCl, 1 mM EDTA, pH 8.0. For custom TE formulations: low-EDTA TE = 10 mM Tris, 0.1 mM EDTA; high-EDTA TE = 10 mM Tris, 10 mM EDTA (for long-term DNA storage in high-nuclease environments). |
| Application Notes / Precautions | For critical applications (single-cell genomics, ancient DNA, low-input NGS), aliquot TE buffer into single-use volumes in a PCR clean hood or biosafety cabinet using sterile filtered pipette tips to maintain sterility and nuclease-free status. Discard any unused portion after opening the aliquot. EDTA absorbs strongly at 220-240 nm and may cause high background in micro-volume spectrophotometry if the reference blank is water vs TE; always blank with the same TE buffer used to dissolve the sample. DNA in TE is more stable than DNA in water: water lacks buffering capacity (pH can drift acidic from dissolved CO2) and nuclease protection. However, pure water is preferred for electroporation (TE salts cause arcing) and mass spectrometry (EDTA adducts). For long-term storage of genomic DNA, use TE pH 8.0 at -80 C; for plasmid DNA, TE pH 8.0 at -20 C is standard. |
| Batch-to-Batch Consistency | pH within 7.98-8.02 for every lot; A260/A280 <0.01; nuclease-free; endotoxin <0.005 EU/mL. |
For research use only, not for clinical use.
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