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| Product Name | ELISA Assay Diluent, 1x Ready-to-Use |
| Catalog No. | ATR-B-YJL-0175 |
| Description | A formulated protein-stabilized diluent designed for dilution of standards, samples, and detection antibodies in sandwich ELISA procedures, minimizing matrix effects and non-specific binding. |
| Intended Use | Diluting recombinant protein standards, serum and plasma samples, and detection antibodies in quantitative sandwich ELISA assays. |
| Principle / Technology | Buffered protein matrix with proprietary stabilizers that maintain protein conformation and reduce adsorption to plastic surfaces. |
| Detection Method | ELISA standard curve linearity verification, spike-recovery analysis |
| Sample Type | Human and mouse serum, plasma, cell culture supernatant, recombinant proteins |
| Performance Range / Specifications | Standard curve linearity R² >0.99; spike recovery 85–115% |
| Sensitivity / LOD | N/A |
| Specificity | No cross-reactivity with common assay interferents |
| Reaction Conditions / Protocol | Use undiluted for sample and standard preparation; compatible with most commercial ELISA kit formats. |
| Components / Formulation | Tris-buffered saline, bovine serum albumin, proprietary protein stabilizers, 0.05% ProClin 300 preservative |
| Storage Conditions | 2–8°C |
| Shelf Life | 18 months |
| Package Specifications | 100 mL, 500 mL |
| Product Form | Liquid, ready to use |
| Quality Control | Standard curve performance verification, intra-lot CV <5%, inter-lot CV <10% |
| Key Features | Ready to use; broad assay compatibility; reduced matrix effects; protein stabilized for extended incubation |
For research use only, not for clinical use.
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