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| Product Name | TBE Electrophoresis Buffer, 10x, pH 8.3 |
| Catalog No. | ATR-B-YJL-0180 |
| Description | A ten-fold concentrated Tris-Borate-EDTA buffer for agarose gel electrophoresis of DNA and RNA, providing high buffering capacity for extended run times at high voltage. |
| Intended Use | Electrophoresis running buffer for separation of DNA fragments and RNA in agarose gels, suitable for analytical and preparative DNA gel applications. |
| Principle / Technology | Boric acid provides buffering capacity; EDTA chelates divalent cations to inhibit nuclease activity; borate ions form complexes with cis-diol groups in sugars. |
| Detection Method | Agarose gel electrophoresis, UV transillumination of ethidium bromide or SYBR-stained gels |
| Sample Type | PCR products, restriction digest fragments, plasmid DNA, genomic DNA, total RNA |
| Performance Range / Specifications | Effective DNA separation 100 bp to 20 kb in 0.5–2% agarose gels |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Dilute 1:10 with deionized water; run at 5–10 V/cm gel length; typical run time 30–90 minutes. |
| Components / Formulation | Tris base 890 mM, boric acid 890 mM, EDTA 20 mM at 10x concentration, pH 8.3 |
| Storage Conditions | 15–30°C; warm to redissolve any precipitate |
| Shelf Life | 24 months |
| Package Specifications | 500 mL, 1 L, 4 L |
| Product Form | Liquid concentrate (10x) |
| Quality Control | pH verification, conductivity check, DNase/RNase free |
| Key Features | High buffering capacity for extended runs; standard for DNA analysis; nuclease free; also available as powder |
For research use only, not for clinical use.
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