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| Product Name | TaqMan Probe qPCR Master Mix |
| Catalog No. | RESK-0069 |
| Description | This 2x ready-to-use master mix is designed for probe-based real-time quantitative PCR. It is formulated with an antibody-based hot-start DNA polymerase that effectively prevents amplification arising from non-specific primer annealing. The optimized recipe combines factors that suppress non-specific PCR amplification with factors that boost amplification efficiency, making the mix well suited for low-concentration templates and enabling reliable standard curves across a wide quantification range. |
| Intended Use | For probe-based real-time quantitative PCR (qPCR) amplification and quantification of DNA templates, including low-concentration templates. |
| Principle / Technology | Antibody-based hot-start DNA polymerase remains inactive at low temperatures, preventing non-specific primer annealing during reaction setup; the polymerase is released upon initial denaturation. The optimized buffer system further suppresses non-specific amplification and enhances amplification efficiency. |
| Detection Method | TaqMan probe-based (hydrolysis probe) real-time fluorescence detection. |
| Sample Type | DNA templates, including low-concentration (low-abundance) templates. |
| Performance Range / Specifications | Amplicon length: 80-300 bp Template GC content: 30%-70% Recommended annealing temperature: 60°C Good standard curves over a wide quantification range Suitable for amplification of low-concentration templates |
| Sensitivity / LOD | Optimized for efficient amplification of low-concentration templates. |
| Specificity | Antibody-mediated hot start combined with specificity-enhancing factors suppresses non-specific amplification caused by primer mis-annealing. |
| Reaction Conditions / Protocol | Supplied as a 2x premix; simply add primers, probe, and template. Recommended annealing temperature: 60°C. Runs on most real-time PCR instruments. |
| Components / Formulation | 2x TaqMan Probe qPCR Master Mix (antibody hot-start DNA polymerase in an optimized reaction buffer system) |
| Storage Conditions | Store at -20°C protected from light. Avoid repeated freeze-thaw cycles; aliquoting is recommended. |
| Shelf Life | 18 months when stored at -20°C. |
| Package Specifications | 1 mL; 5 mL |
| Product Form | Mix (2x premix) |
| Concentration | 2x |
| Shipping Conditions | Shipped with ice packs. |
| Expiration Date / Stability | Valid for 18 months at -20°C; avoid repeated freeze-thaw and store protected from light. |
| Compatibility | Compatible with the vast majority of real-time fluorescence quantitative PCR instruments. |
For research use only, not for clinical use.
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