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| Product Name | TaqMan Multiplex qPCR Master Mix |
| Catalog No. | RESK-0070 |
| Description | This 2x premixed reagent enables multiplex real-time quantitative PCR in a single reaction well. It contains a genetically engineered hot-start Taq DNA polymerase that greatly improves amplification sensitivity and specificity, together with a deeply optimized multiplex buffer system that raises amplification efficiency and promotes efficient amplification of low-concentration templates. The mix is well suited for genotyping and multiplex quantitative gene analysis. |
| Intended Use | For multiplex probe-based real-time quantitative PCR in a single reaction well; suitable for genotyping and multiplex gene quantification analysis. |
| Principle / Technology | Genetically engineered Taq DNA polymerase with antibody-based hot start, combined with a deeply optimized multiplex reaction buffer system for high amplification efficiency and specificity. |
| Detection Method | TaqMan probe-based multiplex real-time fluorescence detection. |
| Sample Type | DNA templates, including low-concentration templates. |
| Performance Range / Specifications | Recommended amplicon length: below 300 bp (overly long products reduce enzyme efficiency; a three-step protocol is recommended if longer products are unavoidable) Recommended reaction volume: 20 μL (5-10 μL is possible, but very small volumes increase pipetting error) Optimized multiplex buffer improves amplification efficiency and supports effective amplification of low-concentration templates |
| Sensitivity / LOD | High amplification sensitivity; promotes effective amplification of low-concentration templates in multiplex reactions. |
| Specificity | The engineered hot-start Taq polymerase and optimized multiplex buffer greatly improve amplification specificity. |
| Reaction Conditions / Protocol | Supplied as a 2x premix; add primers, probes, and template. A 20 μL reaction volume is recommended. Add 50x Low ROX or 50x High ROX reference dye according to the requirements of the qPCR instrument. |
| Components / Formulation | 2x TaqMan Multiplex qPCR Master Mix (1 mL / 5 x 1 mL / 5 mL / 20 x 5 mL, depending on package) 50x Low ROX Reference Dye (40 μL / 200 μL / 200 μL / 20 x 200 μL, depending on package) 50x High ROX Reference Dye (40 μL / 200 μL / 200 μL / 20 x 200 μL, depending on package) |
| Storage Conditions | Store at -20°C. |
| Shelf Life | 18 months when stored at -20°C. |
| Package Specifications | 1 mL; 5 mL; 20 x 5 mL |
| Concentration | 2x (master mix); 50x (ROX reference dyes) |
| Shipping Conditions | Shipped with ice packs. |
| Expiration Date / Stability | Valid for 18 months at -20°C. |
| Compatibility | Compatible with most real-time fluorescence quantitative PCR instruments; separate 50x Low ROX and 50x High ROX reference dyes are provided to match different instrument requirements. |
| Application Notes / Precautions | This master mix is designed for multiplex qPCR; for single-plex qPCR, a dedicated single-plex probe master mix is recommended. A 20 μL reaction volume is recommended to minimize pipetting error; 5 μL or 10 μL volumes are acceptable but very small volumes (e.g., 5 μL) are not recommended. Keep amplicons below 300 bp; for longer products, use a three-step cycling protocol. |
For research use only, not for clinical use.
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