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| Product Name | Taq DNA Polymerase with Mg2+ Buffer |
| Catalog No. | MDKPK-0009 |
| Description | A purified thermostable DNA polymerase supplied with an optimized magnesium-containing reaction buffer for routine PCR applications. |
| Intended Use | Routine PCR, colony PCR and genotyping. |
| Principle / Technology | Thermostable Taq DNA polymerase with 5′→3′ polymerase and 5′→3′ exonuclease activities and no 3′→5′ proofreading activity. |
| Detection Method | Endpoint PCR followed by electrophoretic analysis. |
| Sample Type | DNA templates used in routine PCR, colony PCR and genotyping workflows. |
| Components / Formulation | Taq DNA polymerase and optimized Mg2+-containing PCR buffer. |
| Storage Conditions | Store at −20°C. |
| Package Specifications | 10,000 U. |
| Product Form | DNA polymerase with reaction buffer. |
| Quality Control | Purified preparation reported to be free of endonuclease, exonuclease and bacterial DNA contamination. |
| Key Features | Optimized buffer system; reliable routine amplification; generates PCR products with 3′-A overhangs. |
| Source / Origin | Recombinantly expressed in Escherichia coli carrying the Thermus aquaticus DNA polymerase gene. |
| Compatibility | Suitable for T-vector cloning and rapid recombination-based cloning workflows. |
| Application Notes / Precautions | The enzyme lacks 3′→5′ proofreading activity and produces amplicons with 3′-A overhangs. |
For research use only, not for clinical use.
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