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| Product Name | TAE Electrophoresis Buffer, 50x, pH 8.0 |
| Catalog No. | ATR-B-YJL-0181 |
| Description | A fifty-fold concentrated Tris-Acetate-EDTA buffer for agarose gel electrophoresis of DNA, offering lower ionic strength than TBE and better resolution for large DNA fragments. |
| Intended Use | Electrophoresis running buffer for DNA agarose gel analysis, particularly suited for large fragment separation, gel extraction, and enzymatic reactions from gel slices. |
| Principle / Technology | Acetate provides buffering with lower buffering capacity than borate but improved compatibility with downstream enzymatic manipulations due to reduced borate interference. |
| Detection Method | Agarose gel electrophoresis, DNA purification from gel slices, downstream enzymatic reactions |
| Sample Type | DNA fragments, PCR products, plasmid DNA, restriction digests |
| Performance Range / Specifications | Optimal for 500 bp to 25 kb fragments; resolvable up to 50 kb on low percentage gels |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Dilute 1:50 with deionized water for working concentration; replenish buffer for extended runs. |
| Components / Formulation | Tris base 2 M, glacial acetic acid 1 M, EDTA 50 mM at 50x concentration, pH 8.0 |
| Storage Conditions | 15–30°C |
| Shelf Life | 24 months |
| Package Specifications | 500 mL, 1 L |
| Product Form | Liquid concentrate (50x) |
| Quality Control | pH verification, conductivity check, DNase/RNase free |
| Key Features | 50x concentrate for economy; superior for large fragment separation; compatible with gel extraction kits; lower buffering capacity requires shorter runs or buffer recirculation |
For research use only, not for clinical use.
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