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| Product Name | Streptavidin Magnetic Beads (3 µm) |
| Catalog No. | MAGBEA-0009 |
| Description | Three-micrometer streptavidin-coated superparamagnetic beads engineered for applications demanding ultra-fast magnetic separation while maintaining high biotin binding capacity. The larger particle size enables magnetic capture within 3 seconds, making these beads ideal for automated chemiluminescence immunoassay systems, high-throughput screening, and industrial-scale affinity purification. The high-density streptavidin coating, covalently attached through a multi-point linkage chemistry, provides exceptional biotin-binding capacity and minimal protein leaching under continuous agitation conditions in automated platforms. |
| Intended Use | Capture of biotinylated capture antibodies and nucleic acid probes for automated chemiluminescence immunoassay platforms, high-throughput bead-based ELISA, SELEX aptamer selection, and large-scale biotinylated protein purification. |
| Principle / Technology | Recombinant streptavidin tetramers are covalently immobilized on 3 µm carboxylated polymer beads through multi-point EDC/NHS amide bond formation, followed by a proprietary crosslinking step that eliminates protein leaching under prolonged shear stress. The streptavidin tetramers present four high-affinity biotin-binding pockets (Kd ~10^-15 M per subunit) oriented outward from the bead surface. The 3 µm particle core contains 20-30% magnetite, providing rapid magnetic responsiveness. The large surface area accommodates high-density streptavidin loading with minimal steric hindrance between adjacent tetramers. |
| Detection Method | Coulter counter and DLS for size verification; SEM for morphology; BCA and UV280 for streptavidin density; HABA displacement assay for biotin-binding site quantification; biotin-HRP chemiluminescence assay for functional bead evaluation; automated CLIA platform testing for signal-to-noise ratio and precision |
| Sample Type | Biotinylated monoclonal and polyclonal antibodies, biotinylated antigens, biotinylated oligonucleotide probes, biotinylated peptides, biotin-labeled PCR products; biotin-modified small molecules and haptens for competitive immunoassay formats |
| Performance Range / Specifications | Particle diameter: 3.0 ± 0.3 µm; streptavidin loading: 8-20 µg/mg beads; biotin-binding capacity: 300-1000 pmol free biotin per mg; biotinylated IgG binding: 8-25 µg/mg; biotinylated oligonucleotide: 300-1000 pmol/mg; magnetic separation time: <3 seconds; non-specific binding: <1.5 µg BSA/mg; KD (biotin): ~10^-15 M |
| Sensitivity / LOD | Functional activity detectable at <0.5 µg streptavidin per mg beads; biotinylated analyte detection limits in CLIA format: <1 pg/mL for optimized assays; quantifiable biotin binding at <50 pmol scale; binding linearity maintained across 3-log range of biotinylated ligand inputs |
| Specificity | High-specificity biotin-streptavidin interaction; no binding of non-biotinylated proteins at levels up to 10 mg/mL; no cross-reactivity with biocytin, lipoic acid, or other biotin structural analogs at physiological concentrations; recombinant streptavidin eliminates carbohydrate-associated non-specific binding; minimal lot-to-lot variation in binding specificity |
| Reaction Conditions / Protocol | Wash beads with binding buffer to remove storage stabilizers; add biotinylated ligand in PBS or recommended binding buffer; for antibodies: 5-20 µg biotinylated IgG per mg beads; for oligonucleotides: 50-500 pmol per mg beads; incubate at room temperature for 30-60 minutes with continuous gentle agitation (use rotator to prevent bead settling); wash 3 times with binding buffer containing 0.05% Tween-20; add target analyte and proceed with downstream detection or purification; total bead preparation time: 40-75 minutes |
| Components / Formulation | Streptavidin-coated superparamagnetic polymer beads, 3.0 µm, 10 mg/mL in PBS pH 7.4 with 0.02% sodium azide and 0.1% BSA stabilizer; recombinant core streptavidin (truncated, minimal non-specific binding variant) expressed in E. coli |
| Storage Conditions | 2-8°C; do not freeze; protect from light; store in upright position; vortex thoroughly before use; do not sonicate (may denature streptavidin); avoid contamination with biotin-containing reagents during storage and handling |
| Shelf Life | 18 months at 2-8°C; streptavidin activity >85% at 18 months; protein leaching <0.1% over shelf life; accelerated stability at 37°C for 14 days confirms performance; opened product: 6 months with proper aseptic handling |
| Package Specifications | 1 mL, 5 mL, 10 mL, 50 mL at 10 mg/mL; bulk OEM (100 mL-10 L); custom packaging and concentrations available; trial samples for evaluation; supplied in polypropylene or PET containers |
| Product Form | Dark brown to nearly black suspension; rapid settling requiring vortex before use; homogeneous after 30-60 seconds vortexing; free of protein aggregates and bead clusters; uniform spherical particles by optical microscopy; stable suspension with no sedimentation for >5 minutes after vortexing |
| Quality Control | QC testing per lot: streptavidin density, biotin binding (HABA and biotin-HRP), biotin-IgG capture, biotin-oligo capture, streptavidin leaching (silver stain), non-specific binding, endotoxin, particle size distribution, magnetic separation kinetics, bead count; functional validation on automated CLIA platform with model sandwich immunoassay |
| Key Features | Ultra-fast <3 second magnetic separation for automated platforms; multi-point covalently crosslinked streptavidin eliminates leaching; high biotin binding capacity; recombinant streptavidin with minimal non-specific binding; 3 µm format optimized for CLIA and bead-based ELISA; validated on major automated immunoassay analyzer platforms |
| Purity | Streptavidin >95% by SDS-PAGE; free of avidin; endotoxin <0.05 EU/mg; biotin-free (no detectable competing biotin); streptavidin leaching <0.05% under shear conditions; host cell protein <100 ppm |
| Concentration | 10 mg/mL beads; streptavidin 8-20 µg/mg; biotin binding 300-1000 pmol/mg; exact values in lot-specific certificate; gravimetric bead concentration verified |
| Activity / Unit Definition | Biotin binding: 300-1000 pmol/mg; biotin-IgG: 8-25 µg/mg; biotin-25mer oligo: 300-1000 pmol/mg; activity retained >90% through shelf life; CLIA signal linearity R² >0.99 across 3-log concentration range |
| Molecular Weight | Streptavidin: ~53 kDa (full tetramer) or ~48 kDa (truncated core streptavidin variant, lot-dependent); bead molecular weight not defined for composite material |
| Source / Origin | Recombinant streptavidin expressed in E. coli; magnetic beads are synthetic polystyrene-divinylbenzene-magnetite composite; all components are synthetic or recombinant; certified animal-origin-free; no Streptomyces avidinii-derived components |
| pH Range / Optimal pH | Binding stability: pH 4-10; optimal biotin binding: pH 6.5-8.5; stable in common immunoassay buffers: pH 6-9; denaturation: pH <2 (streptavidin precipitates) or pH >12 (bead degradation); biotin-streptavidin complex stable pH 2-12 |
| Shipping Conditions | Cold pack shipping at 2-8°C; insulated container for extended transit; ambient temperature acceptable <1 week with cold packs; do not freeze; non-hazardous classification; standard courier |
| Expiration Date / Stability | 18 months at 2-8°C; real-time stability: biotin binding >85% at 18 months; protein integrity confirmed by SDS-PAGE; no increase in leaching through expiry; BSA stabilizer maintains streptavidin conformation; after opening: 6 months at 2-8°C |
| Regulatory / Compliance | For research use and IVD component manufacturing; regulatory support documentation available (DMF, CoA, animal-origin-free statement); ISO 13485 manufacturing; CE-marking support for customer IVD registration; batch traceability through unique lot numbers |
| Compatibility | Compatible with all standard biotinylation chemistries (NHS-biotin, sulfo-NHS-biotin, biotin hydrazide, photoactivatable biotin); biotinylated antibodies from all commercial sources; compatible with CLIA substrates (acridinium ester, isoluminol, AMPPD); beads stable in common immunoassay buffers with Tween-20, Triton X-100 up to 0.5%; avoid biotin-containing blocking reagents and serum supplements |
| Recommended Buffer System | Storage: PBS pH 7.4, 0.02% NaN3, 0.1% BSA; binding/wash: PBS or TBS pH 7.4, 0.05% Tween-20; biotin blocking (post-capture): 0.5-1 mM D-biotin in PBS; storage after biotinylated ligand coupling: PBS, 0.02% NaN3, 0.1% BSA; avoid azide-free formulations for long-term use unless using alternative preservative |
| Application Notes / Precautions | BSA stabilizer must be removed by pre-wash if it interferes with biotinylated ligand coupling (BSA may contain trace biotin); calibrate biotinylated antibody amount empirically—overloading may cause aggregation; incubate with continuous rotation to prevent bead settling and ensure uniform coupling; excess free biotin (≥1 mM) is required for competitive elution but is rarely quantitative; for lyophilized assay development, beads can be lyophilized with trehalose or sucrose cryoprotectants after coupling |
| Batch-to-Batch Consistency | Streptavidin density CV <12% inter-lot; biotin binding CV <10%; biotin-IgG capture CV <12%; CLIA signal CV <8% across lots tested on calibrated platform; process capability (Cpk) >1.33 for critical quality attributes; retain samples archived for each production lot |
For research use only, not for clinical use.
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