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| Product Name | Strep-Tactin Magnetic Agarose Beads for >99% Strep-Tag II Protein Purification |
| Catalog No. | IRMGM-0064 |
| Description | Alta DiagnoTech Strep-Tactin magnetic agarose provides mild, high-affinity purification of Strep-tag II proteins while preserving biological activity. |
| Intended Use | Rapid magnetic affinity purification of recombinant Strep-tag II proteins. |
| Principle / Technology | Strep-Tactin affinity binding to the eight-residue Strep-tag II sequence under physiological conditions. |
| Detection Method | Magnetic affinity purification |
| Sample Type | Recombinant proteins carrying the Strep-tag II sequence |
| Performance Range / Specifications | Ligand content is approximately 6 mg Strep-Tactin per mL gel, and reference protein-binding capacity is approximately 7 mg Strep-tag II protein per mL gel. The displayed purification achieved and maintained target-protein purity above 99% across repeated uses. |
| Specificity | Strep-tag II sequence: Trp-Ser-His-Pro-Gln-Phe-Glu-Lys |
| Reaction Conditions / Protocol | Binding and washing: 10 mM Tris-HCl, 150 mM NaCl and 1 mM EDTA at pH 8.0. Elution: 2.5 mM desthiobiotin in binding buffer. |
| Storage Conditions | 2-30 °C; 2-8 °C is recommended for long-term storage |
| Package Specifications | 5, 50 or 250 mL bead formats at 10% v/v and 30-150 µm; a 5 mL purification-kit format is also listed |
| Product Form | Magnetic agarose bead suspension or purification kit |
| Key Features | Mild physiological purification, no tag removal required, purity above 99%, preserved biological activity and reusable affinity media. |
| Purity | >99% in the displayed purification |
| Concentration | 10% v/v bead suspension; 1 mL suspension contains approximately 100 µL settled beads |
| Recommended Buffer System | 10 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH 8.0; 2.5 mM desthiobiotin for elution |
| Product Type | Strep-Tactin affinity magnetic agarose beads |
| Function / Efficacy | Capture and purify Strep-tag II recombinant proteins under physiological conditions. |
| Storage Buffer | 1× PBS containing 0.1% Tween 20 and 0.05% sodium azide |
| Analytes / Targets | Strep-tag II proteins |
| Matrix | Magnetic agarose |
| Particle Size | 30-150 µm |
| Ligand / Bonded Phase | Strep-Tactin |
| Dynamic Binding Capacity | Approximately 7 mg Strep-tag II protein per mL gel; actual capacity depends on the target protein |
| Ligand Density | Approximately 6 mg Strep-Tactin per mL gel |
| Regeneration Conditions | 0.5 M sodium hydroxide or 1 mM HABA |
For research use only, not for clinical use.
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