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| Product Name | Starch Debranching Enzyme (SBE) Activity Assay Kit, 50T/24S |
| Catalog No. | SCAK-YJL-0157 |
| Detection Method | Visible spectrophotometry, Spectrophotometer |
| Storage | Store at 2-8°C, 6 months |
| Intended Use | For research use only. |
| Note | For your safety and health, please wear lab coat, disposable gloves and mask during operation. |
| Product Introduction | Starch debranching enzymes (SBEs), such as isoamylase and pullulanase, hydrolyze the α-1,6-glycosidic linkages in branched polysaccharides like amylopectin and glycogen. They are crucial for determining the final structure and properties of starch granules during biosynthesis, influencing gelatinization, retrogradation, and digestibility. This kit uses a defined branched substrate (e.g., pullulan or phosphorylase limit dextrin). SBE activity is measured by quantifying the increase in reducing ends released after the cleavage of branch points. |
| Product Application | • Research on starch biosynthesis and granule formation in cereal grains, tubers, and leaves. • Studies aiming to modify starch structure and functionality through genetic or biotechnological approaches. • Characterization of microbial debranching enzymes for industrial applications in starch processing. • Investigation of glycogen metabolism in animals and microorganisms. |
| Product Advantages | • Defined Substrate: Uses a well-characterized branched substrate, allowing precise measurement of debranching activity. • Specific Reducing End Assay: Detects the new chain ends created specifically by α-1,6 bond hydrolysis. • Optimized for Plant Extracts: Formulated to handle potential interfering substances in crude plant homogenates. • Fundamental Research Focus: Provides the tool needed to dissect a critical step in starch assembly. |
For research use only, not for clinical use.
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