Sorbitol Dehydrogenase (SDH) Activity Assay Kit, 100T/96S

Sorbitol Dehydrogenase (SDH), with the enzyme classification number EC 1.1.1.14, is a key metabolic enzyme that catalyzes the reversible interconversion between sorbitol and fructose. In this enzymatic reaction, SDH facilitates the dehydrogenation of sorbitol to generate fructose, while simultaneously reducing nicotinamide adenine dinucleotide (NAD⁺) to its reduced form (NADH). This reaction is not only a critical step in regulating intracellular sorbitol levels but also plays a vital role in multiple physiological and pathological processes.
In the context of human health research, SDH activity is closely linked to two major areas: liver function and glucose metabolism-related disorders. Elevated levels of SDH in serum or plasma samples are widely recognized as a specific biomarker for liver damage, as the enzyme is predominantly expressed in liver cells. When liver tissue is injured (e.g., due to toxin exposure, inflammation, or structural damage), SDH is released into the bloodstream, making its activity a reliable indicator for researching liver disease mechanisms—especially when combined with other liver function markers like aminotransferases.
Additionally, SDH is a core enzyme in the polyol pathway, a key alternative pathway for glucose metabolism. Under normal physiological conditions, the polyol pathway is minimally active; however, in states of chronic hyperglycemia (such as in diabetes research models), glucose is converted to sorbitol via aldose reductase, and SDH then catalyzes the conversion of sorbitol to fructose. The accumulation of sorbitol and fructose in tissues (e.g., retinal cells, nerve cells) is closely associated with the development of diabetic complications, including proliferative diabetic retinopathy and diabetic neuropathy. Thus, measuring SDH activity is essential for studying the pathogenesis of these complications and evaluating potential therapeutic interventions.
Beyond human health research, SDH also has significant applications in plant biology studies. In plants, SDH participates in sugar metabolism and stress response mechanisms. For example, under abiotic stress conditions (e.g., drought, salinity, low temperatures), plants adjust their intracellular sugar composition to maintain osmotic balance and protect cellular structures—SDH activity directly influences the conversion between sorbitol and fructose, making it a key target for researching plant stress adaptation.
The Sorbitol Dehydrogenase (SDH) Activity Assay Kit is designed to quantitatively measure SDH activity in various biological samples, providing researchers with a reliable tool to explore the aforementioned physiological and pathological processes. By detecting changes in absorbance (a signal derived from NADH or its coupled reactions) using spectrophotometers or microplate readers, the kit enables accurate quantification of SDH activity, supporting research in fields such as hepatology, diabetes, and plant physiology.