Starch Branching Enzyme (SBE) Activity Assay Kit, 50T/24S
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Starch Branching Enzyme (SBE) Activity Assay Kit, 50T/24S

Cat.No: SCAK-YJL-0092 Datasheet

Specification Quantities

50T/24S:
- +
Product Details Product Overview Related Products
Product Name Starch Branching Enzyme (SBE) Activity Assay Kit, 50T/24S
Catalog No. SCAK-YJL-0092
Detection Method Visible spectrophotometry, Spectrophotometer
Storage Store at 2-8°C, 6 months
Intended Use For research use only.
Note For your safety and health, please wear lab coat, disposable gloves and mask during operation.
Product Characteristics • The main components of this kit are as follows:
Extract solution: Liquid 25 mL×1. Storage at 4°C.
Reagent I: Liquid 20 mL×1. Storage at 4°C.
Reagent II: Powder×2. Storage at 4°C. Add 1 mL of distilled water to each bottle before use. Heat slowly and gradually till boiling to make it fully dissolved.
Reagent III: Liquid 25 mL×1. Storage at 4°C.
• Reagents and equipments required but not provided:
Spectrophotometer, water bath, desk centrifuge, adjustable transferpettor, 1 mL glass cuvette, mortar/homogenizer, ice and distilled water.
Product Introduction Starch branching enzyme (SBE), a key enzyme predominantly found in plants, plays an essential role in amylopectin biosynthesis. Measuring SBE activity is highly valuable for research on starch biosynthesis, screening of high-quality crop varieties, and genetic improvement of crop traits.
The assay is based on the characteristic absorption peak at 660 nm exhibited by the complex formed between starch and iodine. SBE trims the side chains of amylopectin, leading to a reduction in the absorbance of the starch-iodine complex at this wavelength. Within a defined period, the percentage decrease in absorbance serves as a direct indicator of SBE activity.
Product Application Quantifying SBE activity for starch biosynthesis research, crop quality improvement, and plant genetic studies.
Product Advantages • Specific Principle: Precisely measures SBE activity via iodine-starch complex absorbance reduction
• Plant-Optimized: Specifically validated for plant tissue samples
• High Reproducibility: Delivers consistent results with low batch-to-batch variation
• Time-Efficient: Complete protocol with minimal processing steps
• Comprehensive Kit: Includes all necessary reagents and detailed protocol

For research use only, not for clinical use.

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