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| Product Name | Sodium Citrate Buffer, 0.1 M, pH 3.0, Molecular Biology Grade |
| Catalog No. | ATR-B-YJL-0195 |
| Description | 0.1 M sodium citrate buffer solution, pH 3.0, prepared from trisodium citrate dihydrate and citric acid. The citrate buffer system (pKa₁ 3.13, pKa₂ 4.76, pKa₃ 6.40) provides effective buffering across three pH ranges. At pH 3.0, this buffer is near the first pKa, ideal for acid elution of antibodies in affinity chromatography, antigen retrieval for immunohistochemistry at low pH, and pepsin digestion protocols. |
| Intended Use | Low-pH antibody elution from Protein A/G affinity columns; antigen retrieval for IHC (low-pH citrate method); pepsin and acid protease digestion; RNA hydrolysis; zymography renaturation buffer component; heavy metal chelation in biochemical assays. |
| Principle / Technology | Citric acid/sodium citrate buffer system; pKa₁ = 3.13 provides buffering at pH 2.2-3.8; citrate is also a mild chelating agent for divalent cations (Ca²⁺, Mg²⁺); pH 3.0 is below the isoelectric point of most antibodies causing elution from Protein A/G |
| Detection Method | Ready-to-use at 0.1 M; further dilute as needed; for antibody elution: apply to Protein A/G column, incubate 2-5 min, collect fractions into 1/10 volume neutralization buffer (1 M Tris, pH 8.5) |
| Components / Formulation | Trisodium citrate dihydrate (CAS 6132-04-3), citric acid monohydrate (CAS 5949-29-1), ultrapure water; 0.22 µm filtered |
| Storage Conditions | 15-25 °C; keep container tightly closed; sterile-filtered but not guaranteed sterile after opening |
| Shelf Life | 36 months from manufacture date; use within 24 months after opening |
| Package Specifications | 100 mL, 500 mL, 1 L HDPE bottles |
| Product Form | Clear colorless liquid, ready-to-use |
| Key Features | 0.1 M concentration for direct use; pH 3.0 ± 0.05 at 25 °C; suitable for antibody elution, antigen retrieval, and pepsin digestion; mild metal chelation from citrate; molecular biology grade — low heavy metal content; DNase/RNase tested |
| Purity | All components ≥99.5% purity; heavy metals <5 ppm; iron <2 ppm (critical for chelation-sensitive applications); DNase/RNase: not detected |
| Concentration | 0.10 ± 0.01 M total citrate; pH precisely adjusted with citric acid |
| Activity / Unit Definition | pH buffering capacity within ±0.05 pH units of stated value |
| Molecular Weight | Not applicable — inorganic/organic buffer salts |
| Source / Origin | Synthetic, prepared from analytical grade reagents |
| pH Range / Optimal pH | pH 3.0 ± 0.05 at 25 °C; effective buffering range pH 2.2-3.8 |
| Shipping Conditions | Ambient temperature |
| Expiration Date / Stability | 36 months at 15-25 °C; 24 months after opening; discard if microbial growth observed (turbidity); verify pH after prolonged storage |
| Regulatory / Compliance | For laboratory and research use only; RUO; low pH — mild irritant; not classified as dangerous goods; ISO 9001 |
| Compatibility | Compatible with Protein A, Protein G, and Protein A/G affinity resins; compatible with FFPE tissue sections for IHC antigen retrieval; pepsin digestion at pH 2.0-3.0 optimal; citrate is a calcium chelator — avoid in Ca²⁺-dependent enzyme assays; for antigen retrieval, heat to 95-100 °C in citrate buffer for 10-30 minutes |
| Recommended Buffer System | Use as directed; compatible with biological assay systems |
| Application Notes / Precautions | Avoid contamination; use aseptic technique for sterile products; verify pH before critical applications |
| Batch-to-Batch Consistency | pH within ±0.05 units; conductivity within ±5% of reference lot |
For research use only, not for clinical use.
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