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| Product Name | Sodium Acetate Buffer, 3 M, pH 5.2 |
| Catalog No. | ATR-B-YJL-0182 |
| Description | A high-molarity sodium acetate solution at slightly acidic pH, primarily used as a precipitation aid in nucleic acid purification protocols using ethanol precipitation. |
| Intended Use | Providing salt conditions for ethanol precipitation of DNA and RNA, adjusting ionic strength in enzymatic reactions, and serving as a buffer component in various molecular biology procedures. |
| Principle / Technology | Sodium ions neutralize the negative charge of nucleic acid phosphate backbone, reducing solubility; acetate provides pH buffering at the acidic optimum for precipitation efficiency. |
| Detection Method | Spectrophotometric nucleic acid concentration measurement, agarose gel verification of precipitation |
| Sample Type | Purified DNA, RNA, oligonucleotides, PCR cleanup products |
| Performance Range / Specifications | DNA precipitation efficiency >95% for fragments >100 bp |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Add 1/10 volume to nucleic acid solution along with 2.5 volumes of cold absolute ethanol; incubate at -20°C for 30 minutes; centrifuge, wash with 70% ethanol. |
| Components / Formulation | Sodium acetate trihydrate dissolved in ultrapure water, pH adjusted with glacial acetic acid |
| Storage Conditions | 15–30°C |
| Shelf Life | 36 months |
| Package Specifications | 100 mL, 500 mL |
| Product Form | Liquid |
| Quality Control | pH verification, DNase/RNase free, heavy metal analysis |
| Key Features | Nuclease free; standard for ethanol precipitation; compatible with glycogen co-precipitation; DEPC-treated option for RNA work |
For research use only, not for clinical use.
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