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| Product Name | SDS-PAGE Running Buffer, Tris-Glycine, 10x |
| Catalog No. | ATR-B-YJL-0176 |
| Description | A ten-fold concentrated Tris-glycine-SDS electrophoresis buffer providing the ionic conditions for protein separation by molecular weight in denaturing polyacrylamide gel electrophoresis. |
| Intended Use | Electrophoresis running buffer for SDS-PAGE separation of denatured proteins using the Laemmli discontinuous buffer system. |
| Principle / Technology | Glycine ions in the stacking gel create a moving boundary that compresses protein bands, while SDS imparts uniform negative charge proportional to molecular weight, enabling size-based separation in the resolving gel. |
| Detection Method | SDS-PAGE with Coomassie staining, protein molecular weight marker verification |
| Sample Type | Denatured protein lysates, purified proteins, immunoprecipitation eluates |
| Performance Range / Specifications | Protein separation range 10–250 kDa in 4–20% gradient gels |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Dilute 1:10 with deionized water; run at 100–200 V constant voltage; typical run time 45–90 minutes. |
| Components / Formulation | Tris base 250 mM, glycine 1.92 M, SDS 1% w/v at 10x concentration |
| Storage Conditions | 15–30°C; warm to dissolve any SDS precipitate before dilution |
| Shelf Life | 24 months |
| Package Specifications | 500 mL, 1 L, 4 L |
| Product Form | Liquid concentrate (10x) |
| Quality Control | Conductivity verification, pH after dilution, SDS concentration by titration |
| Key Features | Standard Laemmli buffer system; compatible with all polyacrylamide percentages; economical concentrate |
For research use only, not for clinical use.
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