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| Product Name | RIPA Lysis Buffer, 1x, Ready-to-Use |
| Catalog No. | ATR-B-YJL-0178 |
| Description | A complete radioimmunoprecipitation assay lysis buffer containing ionic and non-ionic detergents with protease and phosphatase inhibitors for efficient extraction of total cellular proteins. |
| Intended Use | Extraction of total protein from cultured cells and tissue samples for Western blotting, immunoprecipitation, and protein quantification assays. |
| Principle / Technology | Combination of sodium deoxycholate (ionic), NP-40 (non-ionic), and SDS (denaturing) detergents disrupts cellular membranes and solubilizes cytoplasmic, membrane, and nuclear proteins. |
| Detection Method | BCA or Bradford protein quantification, Western blot analysis of specific markers (cytoplasmic GAPDH, membrane EGFR, nuclear histone H3) |
| Sample Type | Mammalian cultured cells, fresh and frozen tissue biopsies |
| Performance Range / Specifications | Protein yield 2–5 mg/mL from confluent 10 cm dish of HeLa cells |
| Sensitivity / LOD | N/A |
| Specificity | Extracts cytoplasmic, membrane, nuclear, and cytoskeletal protein fractions |
| Reaction Conditions / Protocol | Add cold RIPA buffer to washed cell monolayer or homogenized tissue; incubate on ice 15–30 minutes with intermittent vortexing; centrifuge at 14,000 × g for 15 minutes at 4°C; collect supernatant. |
| Components / Formulation | Tris-HCl pH 7.4 50 mM, NaCl 150 mM, NP-40 1%, sodium deoxycholate 0.5%, SDS 0.1%, EDTA 1 mM, protease inhibitor cocktail, phosphatase inhibitor cocktail |
| Storage Conditions | 2–8°C, protect from light |
| Shelf Life | 12 months; use within 3 months after adding inhibitors |
| Package Specifications | 100 mL, 500 mL |
| Product Form | Liquid, ready to use (inhibitors pre-added) |
| Quality Control | pH verification, lysis efficiency comparison with reference HeLa lysate, protease and phosphatase inhibitor activity verified |
| Key Features | Complete formulation with inhibitors included; extracts total cellular protein; validated for IP and Western blot; compatible with protein assays |
For research use only, not for clinical use.
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