Recombinant Human Tumor Necrosis Factor Receptor Superfamily Member 14 (TNFRSF14/HVEM), Partial, Active
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Recombinant Human Tumor Necrosis Factor Receptor Superfamily Member 14 (TNFRSF14/HVEM), Partial, Active

Cat.No: PTR-HMM-0142 Datasheet

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Product Name Recombinant Human Tumor Necrosis Factor Receptor Superfamily Member 14 (TNFRSF14/HVEM), Partial, Active
Catalog No. PTR-HMM-0142
Description An active recombinant human TNFRSF14 construct representing the partial extracellular region (39-202 aa) and produced in mammalian cells. The protein carries a C-terminal hFc1 tag and is supplied for research use.
Intended Use For research use in functional ELISA; ligand-receptor binding studies.
Principle / Technology Recombinant expression in mammalian cells, followed by purification and functional qualification with sDS-PAGE; functional ELISA.
Detection Method SDS-PAGE; functional ELISA
Sample Type Purified recombinant protein
Performance Range / Specifications ≥90% by SDS-PAGE; molecular weight 48.5 kDa; package options: 10 µg; 20 µg; 50 µg; 100 µg; 200 µg; 500 µg; 1 mg.
Sensitivity / LOD EC50 49.85-79.31 ng/mL or 1.773-3.707 ng/mL, depending on the TNFSF14 assay format.
Specificity Binds human TNFSF14 (LIGHT).
Reaction Conditions / Protocol Use the liquid material as supplied, or briefly centrifuge and reconstitute lyophilized material with sterile deionized water to 0.1-1.0 mg/mL.
Components / Formulation Liquid formulation: Tris- or PBS-based buffer containing 5-50% glycerol; lyophilized formulation: Tris- or PBS-based buffer containing 6% trehalose
Storage Conditions Store at -20°C or -80°C. Prepare working aliquots after opening or reconstitution and avoid repeated freeze-thaw cycles.
Shelf Life Liquid material: up to 6 months at -20°C to -80°C; lyophilized material: up to 12 months at -20°C to -80°C.
Package Specifications 10 µg; 20 µg; 50 µg; 100 µg; 200 µg; 500 µg; 1 mg
Product Form Liquid or lyophilized
Quality Control Purity assessed by SDS-PAGE; functional performance verified in the stated bioassay; endotoxin level <1.0 EU/µg.
Key Features Functionally tested active protein; defined 39-202 aa construct; C-terminal hFc1 tag; ≥90% by SDS-PAGE.
Purity ≥90% by SDS-PAGE
Activity / Unit Definition Functional ELISA confirmed binding to human TNFSF14 with EC50 values of 49.85-79.31 ng/mL and 1.773-3.707 ng/mL in two assay formats.
Molecular Weight 48.5 kDa
Source / Origin Human protein produced in mammalian cells.
Expiration Date / Stability Liquid material: up to 6 months at -20°C to -80°C; lyophilized material: up to 12 months at -20°C to -80°C.
Regulatory / Compliance For research use only; not for clinical diagnosis or treatment.
Compatibility Functional ELISA; ligand-receptor binding studies
Recommended Buffer System Liquid formulation: Tris- or PBS-based buffer containing 5-50% glycerol; lyophilized formulation: Tris- or PBS-based buffer containing 6% trehalose
Application Notes / Precautions For long-term use, store in working aliquots and avoid repeated freeze-thaw cycles. Reconstituted working material may be kept at 4°C for up to one week.
Alternative Names / Synonyms HVEM; CD270; LIGHT receptor; TR2
Lead Time 3-7 business days
Availability Status In Stock
Host Species Human
Tag Information C-terminal hFc1 tag
Amino Acid Sequence LPSCKEDEYPVGSECCPKCSPGYRVKEACGELTGTVCEPCPPGTYIAHLNGLSKCLQCQMCDPAMGLRASRNCSRTENAVCGCSPGHFCIVQDGDHCAACRAYATSSPGQRVQKGGTESQDTLCQNCPPGTFSPNGTLEECQHQTKCSWLVTKAGAGTSSSHWV
UniProt Accession Q92956
Protein Length 164 amino acids
Expression Region 39-202 aa
Endotoxin Level <1.0 EU/µg

For research use only, not for clinical use.

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