- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Recombinant Human Tumor Necrosis Factor Receptor Superfamily Member 14 (TNFRSF14/HVEM), Partial, Active |
| Catalog No. | PTR-HMM-0142 |
| Description | An active recombinant human TNFRSF14 construct representing the partial extracellular region (39-202 aa) and produced in mammalian cells. The protein carries a C-terminal hFc1 tag and is supplied for research use. |
| Intended Use | For research use in functional ELISA; ligand-receptor binding studies. |
| Principle / Technology | Recombinant expression in mammalian cells, followed by purification and functional qualification with sDS-PAGE; functional ELISA. |
| Detection Method | SDS-PAGE; functional ELISA |
| Sample Type | Purified recombinant protein |
| Performance Range / Specifications | ≥90% by SDS-PAGE; molecular weight 48.5 kDa; package options: 10 µg; 20 µg; 50 µg; 100 µg; 200 µg; 500 µg; 1 mg. |
| Sensitivity / LOD | EC50 49.85-79.31 ng/mL or 1.773-3.707 ng/mL, depending on the TNFSF14 assay format. |
| Specificity | Binds human TNFSF14 (LIGHT). |
| Reaction Conditions / Protocol | Use the liquid material as supplied, or briefly centrifuge and reconstitute lyophilized material with sterile deionized water to 0.1-1.0 mg/mL. |
| Components / Formulation | Liquid formulation: Tris- or PBS-based buffer containing 5-50% glycerol; lyophilized formulation: Tris- or PBS-based buffer containing 6% trehalose |
| Storage Conditions | Store at -20°C or -80°C. Prepare working aliquots after opening or reconstitution and avoid repeated freeze-thaw cycles. |
| Shelf Life | Liquid material: up to 6 months at -20°C to -80°C; lyophilized material: up to 12 months at -20°C to -80°C. |
| Package Specifications | 10 µg; 20 µg; 50 µg; 100 µg; 200 µg; 500 µg; 1 mg |
| Product Form | Liquid or lyophilized |
| Quality Control | Purity assessed by SDS-PAGE; functional performance verified in the stated bioassay; endotoxin level <1.0 EU/µg. |
| Key Features | Functionally tested active protein; defined 39-202 aa construct; C-terminal hFc1 tag; ≥90% by SDS-PAGE. |
| Purity | ≥90% by SDS-PAGE |
| Activity / Unit Definition | Functional ELISA confirmed binding to human TNFSF14 with EC50 values of 49.85-79.31 ng/mL and 1.773-3.707 ng/mL in two assay formats. |
| Molecular Weight | 48.5 kDa |
| Source / Origin | Human protein produced in mammalian cells. |
| Expiration Date / Stability | Liquid material: up to 6 months at -20°C to -80°C; lyophilized material: up to 12 months at -20°C to -80°C. |
| Regulatory / Compliance | For research use only; not for clinical diagnosis or treatment. |
| Compatibility | Functional ELISA; ligand-receptor binding studies |
| Recommended Buffer System | Liquid formulation: Tris- or PBS-based buffer containing 5-50% glycerol; lyophilized formulation: Tris- or PBS-based buffer containing 6% trehalose |
| Application Notes / Precautions | For long-term use, store in working aliquots and avoid repeated freeze-thaw cycles. Reconstituted working material may be kept at 4°C for up to one week. |
| Alternative Names / Synonyms | HVEM; CD270; LIGHT receptor; TR2 |
| Lead Time | 3-7 business days |
| Availability Status | In Stock |
| Host Species | Human |
| Tag Information | C-terminal hFc1 tag |
| Amino Acid Sequence | LPSCKEDEYPVGSECCPKCSPGYRVKEACGELTGTVCEPCPPGTYIAHLNGLSKCLQCQMCDPAMGLRASRNCSRTENAVCGCSPGHFCIVQDGDHCAACRAYATSSPGQRVQKGGTESQDTLCQNCPPGTFSPNGTLEECQHQTKCSWLVTKAGAGTSSSHWV |
| UniProt Accession | Q92956 |
| Protein Length | 164 amino acids |
| Expression Region | 39-202 aa |
| Endotoxin Level | <1.0 EU/µg |
For research use only, not for clinical use.
|
There is no product in your cart. |