| Product Name | Glycosylated Serum Protein (GSP) Content Assay Reagent (Microplate Reader) |
| Catalog No. | PTR-HMM-0048 |
| Description | Glycated serum protein (GSP) is a high-molecular-weight ketamine-like substance formed during the non-enzymatic glycation of plasma proteins with glucose. Its concentration is positively correlated with blood glucose levels and remains relatively stable, reflecting the average blood glucose level in the body over the past 1-3 weeks. It can serve as an important indicator for distinguishing stress-induced hyperglycemia and evaluating diabetes control. |
| Application | Serum glucose undergoes a non-enzymatic glycation reaction with the N-terminal amino groups of albumin and other serum protein molecules, forming high-molecular-weight ketamine structures. Under alkaline conditions, these react with nitro-tetrachlorozon blue to produce the purple-red compound methanethiol. The product exhibits a characteristic absorption peak at 530 nm, and the concentration of glycated serum proteins can be quantitatively determined by measuring changes in absorbance. |
| Applicable Instruments | Microplate Reader |
| Number of Testable Samples | 96 Samples |
| Matching | 96-well plate |
| Detection Time | 3 h (96 Samples) |
| Detection Method | NBT |
| Spectral Parameters | 530 nm |
| Signal Response | Incremental |
| Notes | After color development at 37°C, reagent 4 should be added immediately; it is recommended not to prepare too many samples at once to avoid delays in adding reagent 4, which may affect the measurement results; if the A measurement is greater than 1.0 or the ΔA measurement is greater than 0.8, it is recommended to dilute the sample appropriately before measurement and multiply the dilution factor in the calculation formula; If the measured absorbance value is low or close to the blank value, it is recommended to increase the sample volume during sample preparation, re-extract, and then perform the measurement, making corresponding adjustments during calculation. |
For research use only, not for clinical use.
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