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| Product Name | Recombinant Human Somatotropin (GH1), Active |
| Catalog No. | PTR-HMM-0157 |
| Description | An active recombinant human GH1 construct representing the full-length mature protein (27-217 aa) and produced in mammalian cells. The protein carries a N-terminal 10×His tag and C-terminal Myc tag and is supplied for research use. |
| Intended Use | For research use in functional ELISA; LSPR; growth-hormone receptor studies. |
| Principle / Technology | Recombinant expression in mammalian cells, followed by purification and functional qualification with sDS-PAGE; SEC-HPLC; functional ELISA; LSPR. |
| Detection Method | SDS-PAGE; SEC-HPLC; functional ELISA; LSPR |
| Sample Type | Purified recombinant protein |
| Performance Range / Specifications | ≥95% by SDS-PAGE and SEC-HPLC; molecular weight 27.2 kDa; package options: 20 µg; 100 µg; 1 mg. |
| Sensitivity / LOD | PRLR EC50 60.71-69.65 ng/mL; GHR EC50 2.067-25.29 ng/mL; LSPR KD 6.1 nM. |
| Specificity | Binds human prolactin receptor and growth hormone receptor. |
| Reaction Conditions / Protocol | Use the liquid material as supplied, or briefly centrifuge and reconstitute lyophilized material with sterile deionized water to 0.1-1.0 mg/mL. |
| Components / Formulation | 20 mM Tris-HCl, 0.5 M NaCl and 6% trehalose, pH 8.0 |
| Storage Conditions | Store at -20°C or -80°C. Prepare working aliquots after opening or reconstitution and avoid repeated freeze-thaw cycles. |
| Shelf Life | Liquid material: up to 6 months at -20°C to -80°C; lyophilized material: up to 12 months at -20°C to -80°C. |
| Package Specifications | 20 µg; 100 µg; 1 mg |
| Product Form | Liquid or lyophilized |
| Quality Control | Purity assessed by SDS-PAGE; monomeric purity assessed by SEC-HPLC; functional performance verified in the stated bioassay; endotoxin level <1.0 EU/µg. |
| Key Features | Functionally tested active protein; defined 27-217 aa construct; N-terminal 10×His tag and C-terminal Myc tag; ≥95% by SDS-PAGE and SEC-HPLC. |
| Purity | ≥95% by SDS-PAGE and SEC-HPLC |
| Activity / Unit Definition | Functional assays confirmed binding to human prolactin receptor with an EC50 of 60.71-69.65 ng/mL and to growth hormone receptor with EC50 values of 2.067-25.29 ng/mL. LSPR analysis measured a KD of 6.1 nM for growth hormone receptor binding. |
| Molecular Weight | 27.2 kDa |
| Source / Origin | Human protein produced in mammalian cells. |
| pH Range / Optimal pH | pH 8.0 |
| Expiration Date / Stability | Liquid material: up to 6 months at -20°C to -80°C; lyophilized material: up to 12 months at -20°C to -80°C. |
| Regulatory / Compliance | For research use only; not for clinical diagnosis or treatment. |
| Compatibility | Functional ELISA; LSPR; growth-hormone receptor studies |
| Recommended Buffer System | 20 mM Tris-HCl, 0.5 M NaCl and 6% trehalose, pH 8.0 |
| Application Notes / Precautions | For long-term use, store in working aliquots and avoid repeated freeze-thaw cycles. Reconstituted working material may be kept at 4°C for up to one week. |
| Alternative Names / Synonyms | GH-N; growth hormone 1; pituitary growth hormone; somatotropin |
| Lead Time | 1-3 working days |
| Availability Status | In Stock |
| Host Species | Human |
| Tag Information | N-terminal 10×His tag and C-terminal Myc tag |
| Amino Acid Sequence | FPTIPLSRLFDNAMLRAHRLHQLAFDTYQEFEEAYIPKEQKYSFLQNPQTSLCFSESIPTPSNREETQQKSNLELLRISLLLIQSWLEPVQFLRSVFANSLVYGASDSNVYDLLKDLEEGIQTLMGRLEDGSPRTGQIFKQTYSKFDTNSHNDDALLKNYGLLYCFRKDMDKVETFLRIVQCRSVEGSCGF |
| UniProt Accession | P01241 |
| Protein Length | 191 amino acids |
| Expression Region | 27-217 aa |
| Endotoxin Level | <1.0 EU/µg |
For research use only, not for clinical use.
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