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| Product Name | Recombinant Human Signal Regulatory Protein Alpha (SIRPA/CD172a), Partial, Active |
| Catalog No. | PTR-HMM-0168 |
| Description | Human SIRPA extracellular region spanning residues 31-370, produced in mammalian cells with a C-terminal 6xHis-Myc tag. The active protein is validated for interaction with human CD47. |
| Intended Use | CD47-SIRPA interaction studies, functional ELISA, LSPR, receptor-binding research, and assay development. |
| Principle / Technology | A defined recombinant protein region is expressed and purified, then its structural integrity and target-binding function are evaluated using the listed biochemical binding assays. |
| Detection Method | SDS-PAGE; functional ELISA; LSPR |
| Sample Type | Purified recombinant protein |
| Performance Range / Specifications | >=95% by SDS-PAGE; calculated molecular weight 41.8 kDa; package options: 10 ug; 20 ug; 50 ug; 100 ug; 200 ug; 500 ug; 1 mg. |
| Sensitivity / LOD | In functional ELISA, SIRPA bound human CD47 with an EC50 of 58.30-85.04 ng/mL. LSPR measured a CD47 binding affinity of 19.1 nM. |
| Specificity | Binds human CD47. |
| Reaction Conditions / Protocol | Briefly centrifuge the vial before opening. Reconstitute with sterile deionized water to 0.1-1.0 mg/mL. For long-term storage, add 5-50% glycerol as appropriate, divide into aliquots, and freeze. |
| Components / Formulation | Tris- or PBS-based buffer containing 6% trehalose before lyophilization |
| Storage Conditions | Store at -20 C or -80 C upon receipt. Aliquot before repeated use and avoid repeated freeze-thaw cycles. |
| Shelf Life | Typical stability is 6 months for liquid material and 12 months for lyophilized material when stored at -20 C or -80 C. |
| Package Specifications | 10 ug; 20 ug; 50 ug; 100 ug; 200 ug; 500 ug; 1 mg |
| Product Form | Lyophilized powder |
| Quality Control | Purity evaluated by SDS-PAGE; endotoxin tested by LAL assay; biological activity verified by functional ELISA, LSPR. |
| Key Features | Active Human recombinant protein; 31-370 aa expression region; Mammalian cell expression; C-terminal 6xHis-Myc tag; >=95% by SDS-PAGE; low endotoxin. |
| Purity | >=95% by SDS-PAGE |
| Activity / Unit Definition | In functional ELISA, SIRPA bound human CD47 with an EC50 of 58.30-85.04 ng/mL. LSPR measured a CD47 binding affinity of 19.1 nM. |
| Molecular Weight | 41.8 kDa |
| Source / Origin | Human protein produced in Mammalian cell. |
| Expiration Date / Stability | Typical stability is 6 months for liquid material and 12 months for lyophilized material when stored at -20 C or -80 C. |
| Regulatory / Compliance | For research use only; not for clinical diagnosis or treatment. |
| Compatibility | CD47-SIRPA interaction studies, functional ELISA, LSPR, receptor-binding research, and assay development. |
| Recommended Buffer System | Tris- or PBS-based buffer containing 6% trehalose before lyophilization |
| Application Notes / Precautions | Avoid repeated freezing and thawing. A working aliquot may be kept at 4 C for up to one week. |
| Alternative Names / Synonyms | Brain Ig-like molecule with tyrosine-based activation motifs (Bit); CD172 antigen-like family member A; Inhibitory receptor SHPS-1; Macrophage fusion receptor; MyD-1 antigen; Signal-regulatory protein alpha-1; Sirp-alpha-1; Signal-regulatory protein alpha-2; Sirp-alpha-2; Signal-regulatory protein alpha-3; Sirp-alpha-3; p84; CD172a; SHP substrate 1; SHPS-1; BIT; MFR; MYD1; PTPNS1; SHPS1; SIRP |
| Lead Time | 3-7 business days |
| Availability Status | In Stock |
| Host Species | Human |
| Tag Information | C-terminal 6xHis-Myc tag |
| Amino Acid Sequence | EEELQVIQPDKSVLVAAGETATLRCTATSLIPVGPIQWFRGAGPGRELIYNQKEGHFPRVTTVSDLTKRNNMDFSIRIGNITPADAGTYYCVKFRKGSPDDVEFKSGAGTELSVRAKPSAPVVSGPAARATPQHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPVGESVSYSIHSTAKVVLTREDVHSQVICEVAHVTLQGDPLRGTANLSETIRVPPTLEVTQQPVRAENQVNVTCQVRKFYPQRLQLTWLENGNVSRTETASTVTENKDGTYNWMSWLLVNVSAHRDDVKLTCQVEHDGQPAVSKSHDLKVSAHPKEQGSNTAAENTGSNER |
| UniProt Accession | P78324 |
| Protein Length | 340 amino acids |
| Expression Region | 31-370 aa |
| Endotoxin Level | <1.0 EU/ug by LAL assay |
For research use only, not for clinical use.
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