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| Product Name | Recombinant Human Growth/Differentiation Factor 7 (GDF7), Active |
| Catalog No. | PTR-HMM-0121 |
| Description | An active recombinant human GDF7 protein representing full length of mature protein, produced using E. coli and purified for functional protein research. |
| Intended Use | For protein interaction studies, antibody evaluation, assay development, functional characterization and related life-science research. |
| Principle / Technology | Recombinant expression using E. coli, followed by purification and functional qualification. |
| Detection Method | ATDC5 alkaline phosphatase induction assay |
| Sample Type | Purified recombinant protein |
| Performance Range / Specifications | Package options: 10 ug; 100 ug; 500 ug. Purity: >=95% by SDS-PAGE. Functional performance: Biological activity is confirmed by induction of alkaline phosphatase in murine ATDC5 cells, with an ED50 below 1.0 ug/mL and a specific activity above 1,000 IU/mg. |
| Sensitivity / LOD | ED50: <1.0 ug/mL; specific activity: >1,000 IU/mg |
| Specificity | Human GDF7 |
| Reaction Conditions / Protocol | Biological activity is confirmed by induction of alkaline phosphatase in murine ATDC5 cells, with an ED50 below 1.0 ug/mL and a specific activity above 1,000 IU/mg. |
| Components / Formulation | 0.2 um-filtered concentrated solution containing 30% acetonitrile and 0.1% trifluoroacetic acid |
| Storage Conditions | Store at -20 C or -80 C. Prepare working aliquots for repeated use and avoid repeated freeze-thaw cycles. |
| Shelf Life | Liquid: 6 months at -20 C or -80 C. Lyophilized: 12 months at -20 C or -80 C. |
| Package Specifications | 10 ug; 100 ug; 500 ug |
| Product Form | Liquid or lyophilized powder |
| Quality Control | Purity is assessed by the method stated in the specification. Functional performance is qualified by atdc5 alkaline phosphatase induction assay. |
| Key Features | Active recombinant human GDF7; Full length of mature protein; E. coli; >=95% by SDS-PAGE purity; documented functional performance; endotoxin <0.1 EU/ug by LAL assay. |
| Purity | >=95% by SDS-PAGE |
| Activity / Unit Definition | Biological activity is confirmed by induction of alkaline phosphatase in murine ATDC5 cells, with an ED50 below 1.0 ug/mL and a specific activity above 1,000 IU/mg. |
| Molecular Weight | 14.0 kDa |
| Source / Origin | Human; recombinant production using E. coli. |
| Expiration Date / Stability | Liquid material is stable for 6 months at -20 C or -80 C; lyophilized material is stable for 12 months at -20 C or -80 C. |
| Regulatory / Compliance | For research use only; not for clinical diagnosis or treatment. |
| Compatibility | ATDC5 cell-based bioactivity assay; growth factor research; protein interaction studies |
| Recommended Buffer System | 0.2 um-filtered concentrated solution containing 30% acetonitrile and 0.1% trifluoroacetic acid |
| Application Notes / Precautions | Prepare working aliquots when multiple uses are expected. Avoid repeated freeze-thaw cycles and use aseptic handling practices. |
| Alternative Names / Synonyms | BMP12; Bone morphogenetic protein 12; GDF-7; Growth/differentiation factor 7 |
| Lead Time | 5-10 business days |
| Availability Status | In Stock |
| Host Species | Human |
| Tag Information | Tag-Free |
| Amino Acid Sequence | TALAGTRTAQGSGGGAGRGHGRRGRSRCSRKPLHVDFKELGWDDWIIAPLDYEAYHCEGLCDFPLRSHLEPTNHAIIQTLLNSMAPDAAPASCCVPARLSPISILYIDAANNVVYKQYEDMVVEACGCR |
| UniProt Accession | Q7Z4P5 |
| Protein Length | 129 aa |
| Expression Region | 322-450 aa |
| Endotoxin Level | <0.1 EU/ug by LAL assay |
For research use only, not for clinical use.
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