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| Product Name | Rat Gc (Glucagon) ELISA Kit |
| Catalog No. | HTK-YJL-0445 |
| Synonyms | Glicentin ELISA Kit, GC ELISA Kit |
| Species | Rat |
| UniProt ID | P06883 |
| Sample Type | Serum, plasma, cell culture supernatant, cell or tissue lysate, other liquid samples |
| Detection Method | Sandwich ELISA, Double Antibody |
| Detection Wavelength | OD450 |
| Reaction Duration | 4 hours |
| Range | 62.5-4000 pg/ml |
| Sensitivity | 37.5 pg/ml |
| Storage | 2-8°C (Sealed). Don't cryopreserve. |
| Specificity | Specifically binds with Gc, no obvious cross reaction with other analogues. |
| Intended Use | For research use only. |
Glucagon (GC) is a key peptide hormone primarily secreted by the alpha cells of the pancreatic islets, playing a critical role in regulating glucose homeostasis. It acts antagonistically to insulin: when blood glucose levels drop, glucagon stimulates glycogenolysis (breakdown of glycogen) in the liver and gluconeogenesis (production of glucose from non-carbohydrate sources), thereby elevating blood glucose concentrations to maintain physiological balance. Beyond glucose regulation, glucagon also participates in lipid metabolism, promoting lipolysis (breakdown of fats) in adipose tissue, and has been linked to processes in energy metabolism, pancreatic function, and even certain pathological conditions related to metabolic disorders.In life science research, accurate quantification of rat glucagon is essential for studies focused on diabetes, obesity, pancreatic physiology, and metabolic syndrome—especially in preclinical models using rats, which are widely used due to their physiological similarity to humans in metabolic pathways. The Rat Gc (Glucagon) ELISA Kit is designed to address this research need, providing a reliable, efficient tool for detecting and quantifying glucagon levels in various rat-derived biological samples. This kit leverages specific antibody-antigen interactions to ensure precise measurement, supporting researchers in exploring the role of glucagon in normal physiology and disease mechanisms.
Species-specific design: Optimized exclusively for detecting glucagon in rat samples, ensuring no cross-reactivity with glucagon from other species or unrelated analogs in rat biological fluids.
Versatile sample compatibility: Validated for use with multiple rat sample types, including serum, plasma, cell culture supernatant, cell lysate, tissue lysate, and other liquid samples, accommodating diverse research experimental designs.
Sandwich ELISA technology: Adopts a double-antibody sandwich method, which enhances detection specificity and reduces non-specific binding, leading to more accurate and reproducible results compared to less specific assay formats.
Clear detection parameters: Operates at a detection wavelength of OD450 nm (a standard, easy-to-access wavelength in most laboratory microplate readers) and has a reaction duration of 4 hours, balancing efficiency with assay performance.
Well-defined detection range and sensitivity: Offers a detection range of 62.5–4000 pg/ml and a sensitivity of 37.5 pg/ml, enabling quantification of both low and high glucagon concentrations in rat samples, covering the typical physiological range of the hormone.
Convenient storage conditions: Requires only 2–8°C refrigerated storage (no cryopreservation needed for unopened kits), simplifying inventory management and reducing the risk of reagent degradation from repeated freeze-thaw cycles.
High specificity: Rigorously tested to ensure it specifically binds to rat glucagon with no obvious cross-reactivity to other rat-derived peptides or analogs, eliminating false-positive or false-negative results that could compromise research data.
Reliable performance: The sandwich ELISA format and strict manufacturing quality control (including batch-to-batch validation) ensure consistent assay results, allowing researchers to compare data across experiments or laboratories with confidence.
User-friendly workflow: The 4-hour reaction duration is optimized for laboratory efficiency, avoiding overly long incubation times while maintaining assay accuracy; additionally, the kit is designed for standard microplate readers, requiring no specialized equipment.
Broad application scope: Compatible with multiple sample types, making it suitable for a wide range of research areas, such as metabolic biology, pancreatic research, diabetes studies, and cell signaling research involving rat models.
Research-focused design: Explicitly developed for research use, aligning with the needs of academic, pharmaceutical, and biotechnological research teams working on rat-based metabolic or endocrine studies, ensuring the kit meets the rigorous standards of scientific research.
For research use only, not for clinical use.
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