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| Product Name | Rapid High-Sensitivity One-Step RT-qPCR Probe Kit |
| Catalog No. | MDKPK-0030 |
| Description | A rapid probe-based one-step RT-qPCR kit designed for sensitive detection of RNA targets with balanced multiplex amplification and built-in carryover contamination control. |
| Intended Use | Diagnostic pathogen testing, animal pathogen testing, food microbiology testing and rapid quantitative detection of RNA targets. |
| Principle / Technology | A warm-start reverse transcriptase and antibody-regulated Taq DNA polymerase operate in an optimized buffer. A dUTP/thermolabile UDG system removes uracil-containing carryover DNA before amplification. |
| Detection Method | Real-time fluorescent probe-based RT-qPCR. |
| Sample Type | Purified RNA and compatible nasopharyngeal swab lysates. |
| Performance Range / Specifications | Rapid detection within approximately 22 minutes; supports singleplex and multiplex amplification; accepts a large lysate input of up to one-half of the reaction volume in the reported swab-lysate application. |
| Sensitivity / LOD | Designed for stable detection of low-concentration samples; reported Cq values were approximately 1–2 cycles earlier than common comparison reagents in multiple test systems. |
| Specificity | Antibody-mediated hot-start control and the optimized buffer improve amplification specificity, while thermolabile UDG limits carryover contamination. |
| Reaction Conditions / Protocol | Combine RNA template, assay-specific primers and fluorescent probes with the supplied reaction components and run a compatible fast one-step RT-qPCR program. |
| Components / Formulation | One-step RT-qPCR reaction mix containing optimized buffer chemistry and dUTP/UDG carryover-control chemistry, supplied with an enzyme mixture for reverse transcription and hot-start PCR. |
| Package Specifications | 200 reactions; 1,000 reactions; 5,000 reactions. |
| Product Form | Two-component one-step RT-qPCR probe kit. |
| Quality Control | Rapid amplification, multiplex balance, low-template sensitivity and direct-lysate compatibility are evaluated. |
| Key Features | Approximately 22-minute detection; high sensitivity; multiplex compatibility; direct-lysate tolerance; dUTP/UDG contamination control. |
| Compatibility | Suitable for probe-based real-time PCR instruments and assays using RNA templates. |
| Application Notes / Precautions | Use assay-specific primers and probes. Include appropriate positive, negative and no-template controls. |
For research use only, not for clinical use.
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