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| Product Name | Pyruvate (PA) Content Assay Kit (Enzymatic Method) , 100T/48S |
| Catalog No. | SCAK-YJL-0038 |
| Detection Method | Micro-volume method, Spectrophotometer/Microplate Reader |
| Storage | Store at -20°C, 6 months |
| Intended Use | For research use only. |
| Note | For your safety and health, please wear lab coat, disposable gloves and mask during operation. |
| Product Introduction | Pyruvate sits at a critical metabolic junction, linking glycolysis to the TCA cycle, fermentation, and gluconeogenesis. Its concentration is a dynamic indicator of cellular metabolic status. This kit employs a highly specific enzymatic method where pyruvate is converted to lactate by lactate dehydrogenase (LDH) with the simultaneous oxidation of NADH to NAD+. The decrease in absorbance at 340 nm, directly proportional to the pyruvate consumed, provides a precise and direct measurement of pyruvate content in biological fluids, tissues, and culture media. |
| Product Application | • Monitoring glycolytic flux and energy metabolism in cells and tissues. • Research on mitochondrial disorders, cancer metabolism (Warburg effect), and exercise physiology. • Fermentation process monitoring in industrial microbiology and food science. • Measurement of pyruvate in clinical samples for diagnostic purposes. |
| Product Advantages | • Enzymatic Specificity: Utilizes the high specificity of LDH for pyruvate, ensuring minimal interference from other metabolites, resulting in highly accurate and reliable measurements. • Direct Kinetic Measurement: Measures the reaction in real-time (kinetic mode), which is often more accurate than endpoint assays and allows for the detection of a broad concentration range. • No Derivatization Required: Unlike some chemical methods, this enzymatic assay requires no complex sample derivatization, simplifying the workflow and reducing potential errors. • High Reproducibility: The well-defined enzymatic reaction ensures excellent intra- and inter-assay reproducibility, which is critical for comparative studies and time-course experiments. |
For research use only, not for clinical use.
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