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| Product Name | Pyruvate (PA) Content Assay Kit, 100T/96S, 200T/192S |
| Catalog No. | SCAK-YJL-0192 |
| Detection Method | Micro-volume method, Spectrophotometer/Microplate Reader |
| Storage | Store at 2-8°C, 6 months |
| Intended Use | For research use only. |
| Note | For your safety and health, please wear lab coat, disposable gloves and mask during operation. |
| Product Introduction | Pyruvate sits at a critical metabolic junction, linking glycolysis to the TCA cycle, gluconeogenesis, and fermentation. Its concentration reflects the balance between glycolytic flux, mitochondrial pyruvate uptake, and anaplerotic reactions. Accurate pyruvate measurement is essential for studying cellular bioenergetics, metabolic disorders (like mitochondrial diseases), cancer metabolism (Warburg effect), and exercise physiology. This kit uses a highly specific enzymatic reaction where lactate dehydrogenase (LDH) converts pyruvate to lactate while oxidizing NADH to NAD⁺, monitored by the decrease in absorbance at 340 nm. |
| Product Application | • Assessment of glycolytic rate and metabolic flux. • Research on mitochondrial disorders and cellular energy metabolism. • Studies of cancer cell metabolism and the Warburg effect. • Monitoring fermentation processes in industrial microbiology. |
| Product Advantages | • Enzymatic Specificity & Accuracy: The LDH reaction is highly specific for pyruvate, ensuring measurements are free from interference by other keto acids, providing gold-standard accuracy. • Sensitive Kinetic Measurement: The continuous monitoring of NADH oxidation offers high sensitivity and a wide linear range, suitable for both low and high pyruvate concentrations. • Simple Deproteinization Protocol: Includes an efficient method for sample deproteinization to inactivate endogenous LDH and other interfering enzymes prior to analysis. • High-Throughput & Scalable: Available in 100-test and 200-test sizes, optimized for microplate readers to facilitate large-scale metabolic screening studies. |
For research use only, not for clinical use.
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