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| Product Name | Pyruvate Decarboxylase (PDC) Activity Assay Kit, 100T/96S |
| Catalog No. | FAMAK-YJL-0050 |
| Detection Method | Micro-volume method, Spectrophotometer/Microplate Reader |
| Storage | Store at -20°C, 6 months |
| Intended Use | For research use only. |
| Note | For your safety and health, please wear lab coat, disposable gloves and mask during operation. |
| Product Introduction | Pyruvate decarboxylase (PDC) is a thiamine pyrophosphate (TPP)-dependent enzyme that catalyzes the non-oxidative decarboxylation of pyruvate to acetaldehyde and CO₂. This reaction is a key branch point in metabolism, directing carbon flux away from the TCA cycle and towards fermentation pathways, notably ethanol production in yeast and some bacteria. PDC activity is central to bioethanol production and studies of anaerobic metabolism. This kit measures PDC activity by coupling the acetaldehyde produced to the oxidation of NADH via alcohol dehydrogenase. |
| Product Application | • Research on alcoholic fermentation in yeast, bacteria, and plants. • Metabolic engineering for biofuel (ethanol) production. • Studies of anaerobic metabolism and hypoxic response. • Investigation of TPP (vitamin B1)-dependent enzyme mechanisms. |
| Product Advantages | • Specific Coupled Assay: Utilizes alcohol dehydrogenase to specifically detect the acetaldehyde product, ensuring the measured activity is attributable to PDC. • Continuous Kinetic Monitoring: The oxidation of NADH is monitored at 340 nm in real-time, allowing for accurate determination of initial reaction rates. • Includes Essential Cofactor: Provided with thiamine pyrophosphate (TPP), the essential cofactor for PDC activity, ensuring optimal enzyme function. • High-Throughput Format: The 96-well plate compatibility makes this kit ideal for screening multiple conditions, enzyme variants, or inhibitor compounds efficiently. |
For research use only, not for clinical use.
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