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| Product Name | Hydroxymethylglutaryl Coenzyme A Synthase Activity Assay Kit, 50T/48S |
| Catalog No. | FAMAK-YJL-0047 |
| Detection Method | Visible spectrophotometry, Spectrophotometer |
| Storage | Store at -20°C, 6 months |
| Intended Use | For research use only. |
| Note | For your safety and health, please wear lab coat, disposable gloves and mask during operation. |
| Product Introduction | Hydroxymethylglutaryl-CoA (HMG-CoA) synthase catalyzes the condensation of acetoacetyl-CoA with acetyl-CoA to form HMG-CoA. This reaction is a committed step in two critical pathways: mitochondrial ketogenesis (energy production during fasting) and the cytosolic mevalonate pathway (precursor to cholesterol and isoprenoids). This kit measures the reverse reaction for convenience, monitoring the cleavage of HMG-CoA to acetoacetyl-CoA and acetyl-CoA by coupling the release of CoA-SH to a sensitive colorimetric thiol detection system. |
| Product Application | • Research on ketone body metabolism in liver, brain, and other tissues. • Studies of cholesterol biosynthesis and regulation. • Investigation of metabolic disorders like diabetes and hypercholesterolemia. • Drug discovery targeting the mevalonate pathway. |
| Product Advantages | • Sensitive Thiol Detection: Utilizes Ellman's reagent (DTNB) or a similar agent to detect the free CoA-SH released with high sensitivity, enabling activity measurement in complex samples. • Versatile for Isoforms: Can be adapted with specific assay conditions (e.g., buffer, subcellular fractionation) to distinguish between the mitochondrial (ketogenic) and cytosolic (cholesterogenic) isoforms of HMG-CoA synthase. • Well-Defined Substrate: Includes a stable form of HMG-CoA as the substrate, ensuring consistent and reliable reaction initiation. • Comprehensive Background Control: The protocol accounts for non-enzymatic hydrolysis of the substrate, allowing for accurate calculation of specific enzymatic activity. |
For research use only, not for clinical use.
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