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| Product Name | Pyrophosphate: Fructose-6-Phosphate-1-Phosphoric Acid Transferase (PFP) Activity Assay Kit, 100T/96S |
| Catalog No. | SCAK-YJL-0241 |
| Detection Method | Micro-volume method, Spectrophotometer/Microplate Reader |
| Storage | Store at -20°C, 6 months |
| Intended Use | For research use only. |
| Note | For your safety and health, please wear lab coat, disposable gloves and mask during operation. |
| Product Introduction | Pyrophosphate-fructose-6-phosphate 1-phosphotransferase (PFP), also known as pyrophosphate-dependent phosphofructokinase, catalyzes a reversible step in glycolysis: the conversion of fructose-6-phosphate and inorganic pyrophosphate (PPi) to fructose-1,6-bisphosphate and orthophosphate (Pi). It provides an ATP-independent alternative to ATP-dependent phosphofructokinase (PFK), playing a crucial role in energy conservation and metabolic flexibility, especially in plants under stress or low-energy states. This kit measures PFP activity in the glycolytic direction by coupling the production of fructose-1,6-bisphosphate to a colorimetric cascade. |
| Product Application | • Research on glycolytic regulation and energy metabolism in plants, especially under anoxia, phosphate starvation, or biotic stress. • Metabolic studies in parasitic protists (e.g., trypanosomes, giardia) that rely heavily on PFP. • Investigation of bacterial carbohydrate metabolism in certain species. • Screening for potential herbicides or antiparasitic drugs targeting this unique enzyme. |
| Product Advantages | • Specific for PPi Dependency: The assay formulation ensures activity is driven by PPi, distinguishing it from ATP-dependent PFK activity. • High Sensitivity Coupled Assay: The multi-enzyme coupling system amplifies the signal, allowing detection in complex crude extracts. • Large-Scale Format: The 100T/96S configuration supports high-throughput screening applications for drug discovery or functional genomics. • Comprehensive Background Control: Includes protocols to correct for non-specific phosphatase or other interfering activities. |
For research use only, not for clinical use.
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