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| Product Name | Primer/Probe-Compatible One-Tube RT-qPCR Premix |
| Catalog No. | MDKPK-0038 |
| Description | A one-tube RT-qPCR premix that supports advance addition of primers and probes, enabling a simplified add-sample-and-run workflow. |
| Intended Use | Molecular point-of-care testing, pathogen testing, fusion-gene detection, DNA/RNA co-detection and gene-expression analysis. |
| Principle / Technology | One-step reverse transcription and hot-start probe qPCR are performed with a concentrated premix formulated for stable primer/probe incorporation. |
| Detection Method | Fluorescent probe-based real-time RT-qPCR. |
| Sample Type | RNA, DNA/RNA mixtures and other compatible nucleic-acid templates. |
| Performance Range / Specifications | 200, 1,000 or 10,000 reactions at 20 µL per reaction; supports advance primer/probe premixing and balanced DNA/RNA amplification. |
| Specificity | Hot-start qPCR chemistry reduces nonspecific amplification during setup and temperature ramping. |
| Reaction Conditions / Protocol | Add assay-specific primers and probes to the premix in advance where appropriate. Add template immediately before amplification and run a compatible one-step RT-qPCR program. |
| Components / Formulation | One-tube one-step RT-qPCR premix containing reverse-transcription and hot-start PCR components in an optimized buffer. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 200 reactions; 1,000 reactions; 10,000 reactions (20 µL per reaction). |
| Product Form | One-tube RT-qPCR probe premix. |
| Quality Control | Primer/probe premix stability and DNA/RNA co-detection performance are evaluated. |
| Key Features | Advance primer/probe premixing; add-sample-and-run workflow; DNA/RNA co-detection; point-of-care compatibility. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Suitable for probe-based real-time PCR systems and molecular point-of-care reagent development. |
| Application Notes / Precautions | Validate primer/probe concentrations and working-reagent stability for each assay configuration. |
For research use only, not for clinical use.
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