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| Product Name | Polysaccharide Content Assay Kit, 50T/48S |
| Catalog No. | SCAK-YJL-0030 |
| Detection Method | Visible spectrophotometry |
| Storage | Store at 2-8°C |
| Intended Use | For research use only. |
| Note | For your safety and health, please wear lab coat, disposable gloves and mask during operation. |
| Product Introduction | Polysaccharides, including starch, cellulose, hemicellulose, and various gums, are major components of plant biomass, microbial capsules, and food products. This kit utilizes the classic and robust phenol-sulfuric acid method. In concentrated sulfuric acid, polysaccharides are hydrolyzed to monosaccharides, which are then dehydrated to furfural derivatives. These compounds react with phenol to form a stable orange-gold color, the intensity of which is proportional to the total polysaccharide concentration, measured spectrophotometrically. |
| Product Application | • Determination of total carbohydrate/polysaccharide content in plant tissues, algae, and fungi. • Analysis of exopolysaccharide production in bacterial and fungal cultures. • Quality control in food, pharmaceutical, and cosmetic industries (e.g., for gums, thickeners). • Monitoring polysaccharide extraction yields and purification processes. |
| Product Advantages | • Broad Specificity for Carbohydrates: Reacts with a wide range of neutral sugars (hexoses, pentoses, uronic acids) making it excellent for measuring total polysaccharide content from diverse sources. • Rapid & High-Throughput: The microplate-based format allows for the simultaneous processing of many samples in under two hours, significantly faster than gravimetric methods. • Cost-Effective & Simple: Uses common laboratory chemicals (phenol, sulfuric acid) in a pre-optimized protocol, offering a very economical solution without the need for expensive enzymes. • Includes Standard Curve: Provides D-glucose as a standard for calibration, though users can select other relevant monosaccharide standards for more specific quantitation if the polysaccharide composition is known. |
For research use only, not for clinical use.
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