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| Product Name | Plant Total Protein Extraction Kit |
| Catalog No. | REKI-0005 |
| Description | Plant tissues resist conventional protein extraction because cell walls, phenolics and proteases conspire to degrade yield. This kit grinds plant material thoroughly under liquid nitrogen and then extracts released proteins under denaturing conditions with a dedicated reagent system. Reagent A carries sodium orthovanadate, sodium fluoride, EDTA and leupeptin, so proteolysis is suppressed from the first step and protein integrity is maximised. The kit recovers total protein including membrane, nuclear, cytoplasmic and rare species from plant cells, tissues or protoplasts. |
| Intended Use | Denaturing total protein extraction from plant tissues, cells and protoplasts for SDS-PAGE, Western blotting and immunoprecipitation |
| Principle / Technology | Liquid nitrogen grinding plus denaturing reagent chemistry releases the complete plant proteome while inhibitors protect it |
| Detection Method | Total plant protein including membrane, nuclear, cytoplasmic and rare species |
| Sample Type | Plant tissues, plant cells and protoplasts |
| Performance Range / Specifications | Denaturing extraction maximises recovery; inhibitor blend preserves integrity through the workflow; 100 or 200 preparations per kit |
| Specificity | Plant-optimised chemistry addressing cell walls and endogenous protease load |
| Reaction Conditions / Protocol | Grind material under liquid nitrogen, extract with the denaturing reagent system, clarify and proceed to electrophoresis or blotting |
| Components / Formulation | Reagent A (stored aliquoted at -20°C) and reagent B; sized for 100 or 200 preparations |
| Storage Conditions | Reagent A aliquoted at -20°C; reagent B at -20°C |
| Shelf Life | 12 months |
| Package Specifications | 100 tests; 200 tests |
| Product Form | Ready-to-use reagent set |
| Quality Control | Extraction yield and inhibitor content verified on each lot |
| Key Features | Liquid nitrogen workflow tuned to plant material; denaturing chemistry for complete recovery; inhibitors pre-blended; covers membrane and nuclear proteins |
| Source / Origin | Formulated kit |
| Shipping Conditions | Wet ice |
| Expiration Date / Stability | 12 months frozen |
| Regulatory / Compliance | For research use only |
| Compatibility | Extracts feed directly into SDS-PAGE, Western blotting and immunoprecipitation |
| Recommended Buffer System | Denaturing extraction reagents with sodium orthovanadate, sodium fluoride, EDTA and leupeptin in reagent A |
| Application Notes / Precautions | Maintain liquid nitrogen handling safety; keep reagent A aliquoted to avoid freeze-thaw cycles |
| Batch-to-Batch Consistency | Lot-numbered packaging; lot record retained for traceability |
| Starting Material / Tissue Site | Plant tissues, plant cells or protoplasts supplied by the user |
| Delivery Standard | Standard catalogue shipment, packaging integrity verified before dispatch |
| Readouts / Downstream Assays | SDS-PAGE, Western blotting, immunoprecipitation of plant proteins |
For research use only, not for clinical use.
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