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Cat.No: DB-0509 Datasheet Instruction for Use
Specification Quantities
Price $1182.36–1970.60
| Product Name | Interleukin 6 (IL-6) Test Kit |
| Catalog No. | DB-0509 |
| Description | The kit assay human IL-6 level in the sample, use purified human IL-6 antibody to coat microtiter plate wells, make solid-phase antibody, then add IL-6 to the wells, Combined antibody which With HRP labeled, become antibody-antigen-enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IL-6 in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
| Kit Components | User manual：1 Closure plate membrane：2 Sealed bags：1 Microelisa stripplate：1 Standard：0.3ml×6 bottle HRP-Conjugate reagent：5ml×1 bottle Sample diluent：3ml×1 bottle Chromogen Solution A：3ml×1 bottle Chromogen Solution B：3ml×1 bottle Stop Solution：3ml×1 bottle 20× Wash solution：15ml×1 bottle |
| Sample Type | Human serum, plasma, tissue homogenates and other biological fluids |
| Detection Range | 1.5 pg/mL - 48 pg/mL |
| Sample Preparation | Serum. Coagulation at room temperature 10-20 mins, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. Plasma. Use suited EDTA or citrate plasma as an anticoagulant, mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. Urine. Collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The operation of hydrothorax and cerebrospinal fluid reference to it. Cell Culture Supernatant. Detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, detect the composition of cells. Dilut cell suspension with PBS, cell concentration reached 1 million/ml, repeated freeze-thaw cycles, damage cells and release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. Tissue Samples. After cutting samples, check the weight, add PBS, rapidly frozen with liquid nitrogen, maintain samples at 2-8°C after melting, add PBS, homogenized by hand or grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant. |
| Test Procedure | Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well. Add sample: Set blank wells separately (blank comparison wells don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix. Add enzyme: Add HRP-Conjugate reagent 100μl to each well, except blank well. Incubate: After closing plate with Closure plate membrane ,incubate for 60 min at 37°C. Configurate liquid: 20-fold wash solution diluted 20-fold with distilled water and reserve. Washing: Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat. Color: Add chromogen solution A 50ul and chromogen solution B to each well, evade the light preservation for 15 min at 37°C Stop the reaction: Add stop solution 50μl to each well, stop the reaction(the blue color change to yellow color). Assay: Take blank well as zero , read absorbance at 450nm after Adding Stop Solution and within 15min. |
| Results | Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density. |
| Storage and Transportation | Storage: 2-8°C. Validity: 6 months. |
For research use only, not for clinical use.
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